Trial reportJournal of thrombosis and thrombolysis2014
Propensity for young reticulated platelet recruitment into arterial thrombi.
Trial report in Journal of thrombosis and thrombolysis, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 70 citations in OpenAlex.
- Immature platelet fraction and high-on treatment platelet reactivity with ticagrelor in patients with acute coronary syndromes.Journal of thrombosis and thrombolysis · 2016Trial
- Gradual increase in thrombogenicity of juvenile platelets formed upon offset of prasugrel medication.Haematologica · 2015Trial
- Review
- Reticulated platelets in coronary artery disease: a multidimensional approach unveils prothrombotic signalling and novel therapeutic targets.European heart journal · 2025Article
- The Role of Immature Platelet Fraction and Reticulated Platelets in Stroke Monitoring and Outcome Prognosis: A Systematic Review.Journal of clinical medicine · 2025Review
- Changes in platelet maturity and reactivity following acute ST-segment elevation myocardial infarction.Research and practice in thrombosis and haemostasis · 2025Article
- Differential Effects of Erythropoietin Administration and Overexpression on Venous Thrombosis in Mice.Thrombosis and haemostasis · 2024Article
- Article
- Multiparameter phenotyping of platelets and characterization of the effects of agonists using machine learning.Research and practice in thrombosis and haemostasis · 2024Article
- Article
- Platelets and the Atherosclerotic Process: An Overview of New Markers of Platelet Activation and Reactivity, and Their Implications in Primary and Secondary Prevention.Journal of clinical medicine · 2023Review
- Advanced Flow Cytometry Using the SYTO-13 Dye for the Assessment of Platelet Reactivity and Maturity in Whole Blood.Methods and protocols · 2023Article
- Immature platelet fraction in cardiovascular diagnostics and antiplatelet therapy monitoring.Cardiology journal · 2023Review
- Temporal in vivo platelet labeling in mice reveals age-dependent receptor expression and conservation of specific mRNAs.Blood advances · 2022Article
- Proteome and functional decline as platelets age in the circulation.Journal of thrombosis and haemostasis : JTH · 2021Article
- Lower Antiplatelet Effect of Aspirin in Essential Thrombocythemia than in Coronary Artery Disease.TH open : companion journal to thrombosis and haemostasis · 2021Article
- Reticulated Platelets-Which Functions Have Been Established by In Vivo and In Vitro Data?Cells · 2021Review
- Integrative Genomic Analysis Reveals Four Protein Biomarkers for Platelet Traits.Circulation research · 2020Article
- It's reticulated: the liver at the heart of atherosclerosis.The Journal of endocrinology · 2018Review
- Obesity and Altered Aspirin Pharmacology.Clinical pharmacokinetics · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Atherosclerosis reduces platelet survival and thereby increases the percentage of younger platelets in the circulation assuming steady-state thrombocytopoiesis. We hypothesized that younger platelets have an increased propensity for arterial thrombus participation compared to older counterparts. Platelet-rich thrombi were generated by perfusing human heparinized whole blood from normal donors over arterial cross-sections under shear conditions (3,350 s(-1)) corresponding to significant coronary artery stenosis using a perfusion chamber. Harvested thrombi were disaggregated, stained with thiazole orange, anti-integrin β3, glycoprotein (GP) Ibα, GP IX and P-selectin, and compared to paired whole blood samples from the same donor by flow cytometry. Thiazole orange staining intensity provides a measure of platelet m-RNA content and age. Thiazole orange staining intensity (MN ± SEM) of platelets harvested from thrombi (62 ± 13) was twofold greater compared to paired intra-individual whole blood samples (31 ± 1). Integrin β3 receptor density was also greater for thrombus platelets (12.0 ± 1.0) compared to whole blood platelets (7.0 ± 0.6; p < 0.0001). GPs Ibα and IX were reduced from thrombus platelets possibly reflecting shedding. Younger "reticulated" platelets appear to have a greater propensity for thrombus participation under shear conditions of coronary artery stenosis compared to older counterparts. This predisposition may be explained by an increased receptor density of integrin β3 in younger platelets. By this mechanism, the atherosclerotic process may enhance the individual propensity for arterial thrombosis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.