ArticleInternational orthopaedics2013
Dexamethasone induces osteogenesis via regulation of hedgehog signalling molecules in rat mesenchymal stem cells.
Article in International orthopaedics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- Enhancing in vitro osteogenic differentiation of mesenchymal stem cells via sustained dexamethasone delivery in 3D-Printed hybrid scaffolds based on polycaprolactone-nanohydroxyapatite/alginate-gelatin for bone regeneration.Journal of biological engineering · 2025Article
- Dexamethasone-Functionalized PLLA Membranes: Effects of Layer-by-Layer Coating and Electrospinning on Osteogenesis.Bioengineering (Basel, Switzerland) · 2025Article
- Cortisol regulates neonatal lung development via Smoothened.Respiratory research · 2025Article
- Research progress on the hedgehog signalling pathway in regulating bone formation and homeostasis.Cell proliferation · 2022Review
- Article
- Combining Smoothened Agonist and NEL-Like Protein-1 Enhances Bone Healing.Plastic and reconstructive surgery · 2017Article
- A novel bioactive osteogenesis scaffold delivers ascorbic acid, β-glycerophosphate, and dexamethasone in vivo to promote bone regeneration.Oncotarget · 2017Article
- The role of growth factors in stem cell-directed chondrogenesis: a real hope for damaged cartilage regeneration.International orthopaedics · 2015Review
- Dexamethasone in osteogenic medium strongly induces adipocyte differentiation of mouse bone marrow stromal cells and increases osteoblast differentiation.BMC cell biology · 2015Article
- Genistein attenuates glucocorticoid-induced bone deleterious effects through regulation Eph/ephrin expression in aged mice.International journal of clinical and experimental pathology · 2015Article
- Icariin attenuates glucocorticoid-induced bone deteriorations, hypocalcemia and hypercalciuria in mice.International journal of clinical and experimental medicine · 2015Article
- Efficient derivation of osteoprogenitor cells from induced pluripotent stem cells for bone regeneration.International orthopaedics · 2014Article
- Role of mesenchymal stem cells in bone regeneration and fracture repair: a review.International orthopaedics · 2013Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeHedgehog signalling plays an important role during the development of tissues and organs, including bone and limb. Dexamethasone (DEX), a synthetic and widely used glucocorticoid, affects osteogenesis of bone marrow mesenchymal stem cells (MSCs), while the signalling pathway by which DEX affects osteoblast differentiation remains obscure. This study aimed to investigate expressions of hedgehog signalling molecules Shh, Ihh and Gli1 during DEX-induced osteogenesis of rat MSCs in vitro.
methodsDEX promoted osteoblast differentiation of MSCs at 10(-8) mol/L from seven days to 21 days, demonstrated by enhancing alkaline phosphatase (ALP) activity and osteoblast-associated marker type I collagen expression during osteoblastic differentiation. Gene and protein expressions of hedgehog signalling molecules, Shh, Ihh and Gli1 were tested by RT-PCR and western blot analysis during osteoblast differentiation.
resultsShh expression was increased compared to the control while Ihh and Gli1 expressions were decreased on both mRNA and protein level during DEX-induced osteoblast differentiation of MSCs from seven days to 21 days. Altogether, these data demonstrate that DEX can enhance Shh expression via a Gli1-independent mechanism during osteoblast differentiation of MSCs.
conclusionsThese results indicate that different patterns of hedgehog signalling are involved in DEX-induced osteogenesis and these findings provide insights into the mechanistic link between glucocorticoid-induced osteogenesis and hedgehog signalling pathway.
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