ArticleInternational orthopaedics2013
Enhanced migration of human bone marrow stromal cells in modified collagen hydrogels.
Article in International orthopaedics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review.Journal of orthopaedic surgery and research · 2017Pooled it
- Comment on Roessler et al.: Short-term follow up after implantation of a cell-free collagen type I matrix for the treatment of large cartilage defects of the knee.International orthopaedics · 2016Article
- Collagen type I coating stimulates bone regeneration and osteointegration of titanium implants in the osteopenic rat.International orthopaedics · 2015Article
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Authors and funding
8 authors.
Funding
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Abstract
purposeCollagen I hydrogels are widely used as scaffolds for regeneration of articular cartilage defects. We hypothesised that ingrowth might be improved by removing the superficial layer of a compressed hydrogel. The control group consisted of the original unmodified product.
methodsThe migration of human bone marrow stromal cells (hBMSCs) into the hydrogel was evaluated by confocal microscopy. We quantified the DNA concentration of the hydrogel for each group and time point and evaluated the chondrogenic differentiation of cells.
resultsAfter one week, the detectable amount of cells at the depth of 26-50 μm was significantly higher in the modified matrix (MM) than in the non-modified matrix (NM) (p = 0.011). The maximum depth of penetration was 75 μm (NM) and 200 μm (MM). After three weeks, the maximum depth of penetration was 175 μm (NM) and 200 μm (MM). Likewise, at a depth of 0-25 μm the amount of detectable cells was significantly higher in the MM group (p = 0.003). After 14 days, the concentration of DNA was significantly higher in the samples of the MM than in the control group (p = 0.000). Staining of histological sections and labelling with collagen II antibodies showed that a chondrogenic differentiation of cells in the scaffold can occur during in vitro cultivation.
conclusionsRemoving the superficial layer is essential to ensuring proper ingrowth of cells within the compressed hydrogel. Compressed hydrogels contribute better to cartilage regeneration after surface modification.
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