Evidence map›Paper›PMID 23644881›Full record

ArticleExperimental & applied acarology2013

Isolation and characterization of ten polymorphic microsatellite loci in Ixodes arboricola, and cross-amplification in three other Ixodes species.

N Van Houtte, A R Van Oosten, K Jordaens, E Matthysen, T Backeljau, D J A Heylen

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Article in Experimental & applied acarology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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3 · Its place in the literature

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2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

N Van HoutteEvolutionary Ecology Group, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium.
A R Van Oosten
K Jordaens
E Matthysen
T Backeljau
D J A Heylen

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We characterized ten polymorphic microsatellite loci from the tree-hole tick, Ixodes arboricola. Loci were screened in 11-18 individuals from three Belgian populations and five to ten alleles were found at each locus. Seven loci did not show deviations from Hardy-Weinberg equilibrium conditions and there were no indications for null alleles at these loci. The three other loci showed significant heterozygote deficiencies in at least one population, and a high potential for the occurrence of null alleles. We observed no effect of potential host DNA on the scoring of the microsatellites. Cross-amplification of the microsatellites was tested in eight specimens of three congeneric species: I. ricinus, I. hexagonus and I. frontalis. Depending on the species, six or seven of the loci were amplified in ≥ 4 of the 8 specimens and were polymorphic in each of these species (except for Ixaf 11 in I. frontalis and I. ricinus). These loci thus provide a tool for population genetic analysis of I. arboricola. The suitability of these markers needs to be further investigated in its congeners.

Indexed as

Microsatellite RepeatsPolymorphism, GeneticAllelesAnimalsDNAIxodesSpecies SpecificityDNA

Identifiers

PMID23644881

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.