ArticleEpigenetics2013
High quality methylome-wide investigations through next-generation sequencing of DNA from a single archived dry blood spot.
Article in Epigenetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A methylation study implicates the rewiring of brain neural circuits during puberty in the emergence of sex differences in depression symptoms.Journal of child psychology and psychiatry, and allied disciplines · 2022Pooled it
- Longitudinal Analysis of Estrogen Receptor Gene Methylation, Estradiol, and Depressive Symptoms During the Perinatal Period.Molecular neurobiology · 2025Article
- A methylation study of long-term depression risk.Molecular psychiatry · 2020Article
- DifferentialInternational journal of molecular sciences · 2020Article
- Impact ofFrontiers in genetics · 2020Article
- Sleep and Methylation of Estrogen Receptor Genes,Nature and science of sleep · 2020Article
- DNA Methylation in Healthy Older Adults With a History of Childhood Adversity-Findings From the Women 40+ Healthy Aging Study.Frontiers in psychiatry · 2019Article
- Assessment of dried blood spots for DNA methylation profiling.Wellcome open research · 2019Article
- Association betweenEpigenetics · 2018Article
- A MBD-seq protocol for large-scale methylome-wide studies with (very) low amounts of DNA.Epigenetics · 2017Article
- Genome-Wide Epigenetic Studies in Human Disease: A Primer on -Omic Technologies.American journal of epidemiology · 2016Article
- Genome-wide DNA methylation profiling with MeDIP-seq using archived dried blood spots.Clinical epigenetics · 2016Article
- Development of DNA confirmatory and high-risk diagnostic testing for newborns using targeted next-generation DNA sequencing.Genetics in medicine : official journal of the American College of Medical Genetics · 2015Article
- Article
- Longitudinal epigenetic variation of DNA methyltransferase genes is associated with vulnerability to post-traumatic stress disorder.Psychological medicine · 2014Article
- Genomic methylation patterns in archaeological barley show de-methylation as a time-dependent diagenetic process.Scientific reports · 2014Article
- Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling.BMC biotechnology · 2014Article
- The promise and challenges of blood spot methylomics.Epigenetics · 2013Review
- Analysis of epigenetic changes in survivors of preterm birth reveals the effect of gestational age and evidence for a long term legacy.Genome medicine · 2013Article
Corrections and comments
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Authors and funding
6 authors.
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Abstract
The potential importance of DNA methylation in the etiology of complex diseases has led to interest in the development of methylome-wide association studies (MWAS) aimed at interrogating all methylation sites in the human genome. When using blood as biomaterial for a MWAS the DNA is typically extracted directly from fresh or frozen whole blood that was collected via venous puncture. However, DNA extracted from dry blood spots may also be an alternative starting material. In the present study, we apply a methyl-CpG binding domain (MBD) protein enrichment-based technique in combination with next generation sequencing (MBD-seq) to assess the methylation status of the ~27 million CpGs in the human autosomal reference genome. We investigate eight methylomes using DNA from blood spots. This data are compared with 1,500 methylomes previously assayed with the same MBD-seq approach using DNA from whole blood. When investigating the sequence quality and the enrichment profile across biological features, we find that DNA extracted from blood spots gives comparable results with DNA extracted from whole blood. Only if the amount of starting material is ≤ 0.5µg DNA we observe a slight decrease in the assay performance. In conclusion, we show that high quality methylome-wide investigations using MBD-seq can be conducted in DNA extracted from archived dry blood spots without sacrificing quality and without bias in enrichment profile as long as the amount of starting material is sufficient. In general, the amount of DNA extracted from a single blood spot is sufficient for methylome-wide investigations with the MBD-seq approach.
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