ArticleAnnals of botany2013
High cytokinin levels induce a hypersensitive-like response in tobacco.
Article in Annals of botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.
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Who cites it
28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 62 citations in OpenAlex.
- Abiotic Stress in Crop Production.International journal of molecular sciences · 2023Pooled it
- Hydrogen Peroxide: Its Role in Plant Biology and Crosstalk with Signalling Networks.International journal of molecular sciences · 2018Pooled it
- Dexamethasone-inducible LhGR/pOp system: simple and flexible spatiotemporal control of gene expression.Planta · 2026Review
- Preserving the adaptive salt stress response activity of a tissue-specific promoter with modulating activity.Journal, genetic engineering & biotechnology · 2024Article
- Plant Stomata: An Unrealized Possibility in Plant Defense against Invading Pathogens and Stress Tolerance.Plants (Basel, Switzerland) · 2023Review
- Hormonal crosstalk controls cell death induced by kinetin in roots of Vicia faba ssp. minor seedlings.Scientific reports · 2023Article
- Isolation and characterization of drought and ABA responsive promoter of a transcription factor encoding gene from rice.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Cytokinin Deficiency Alters Leaf Proteome and Metabolome during Effector-Triggered Immunity inPlants (Basel, Switzerland) · 2022Article
- Light Quality Modulates Plant Cold Response and Freezing Tolerance.Frontiers in plant science · 2022Article
- Isopentenyltransferases as master regulators of crop performance: their function, manipulation, and genetic potential for stress adaptation and yield improvement.Plant biotechnology journal · 2021Review
- Abscisic Acid-Induced Stomatal Closure: An Important Component of Plant Defense Against Abiotic and Biotic Stress.Frontiers in plant science · 2021Review
- Transcriptomic and Metabolomic Analyses Provide Insights Into an Aberrant Tissue of Tea Plant (Frontiers in plant science · 2021Article
- Barley Root Proteome and Metabolome in Response to Cytokinin and Abiotic Stimuli.Frontiers in plant science · 2020Article
- Role of Cytokinins for Interactions of Plants With Microbial Pathogens and Pest Insects.Frontiers in plant science · 2019Review
- Accumulation of Catechin and Proanthocyanidins in Black Poplar Stems After Infection byFrontiers in plant science · 2019Article
- Role of Cytokinins in Senescence, Antioxidant Defence and Photosynthesis.International journal of molecular sciences · 2018Review
- Cytokinin at the Crossroads of Abiotic Stress Signalling Pathways.International journal of molecular sciences · 2018Review
- The cereal pathogen Fusarium pseudograminearum produces a new class of active cytokinins during infection.Molecular plant pathology · 2018Article
- Genome-wide identification of new reference genes for RT-qPCR normalization in CGMMV-infectedPeerJ · 2018Article
- In silico analysis of the grapefruit sRNAome, transcriptome and gene regulation in response to CTV-CDVd co-infection.Virology journal · 2017Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsCytokinins are positive regulators of shoot development. However, it has previously been demonstrated that efficient activation of the cytokinin biosynthesis gene ipt can cause necrotic lesions and wilting in tobacco leaves. Some plant pathogens reportedly use their ability to produce cytokinins in disease development. In response to pathogen attacks, plants can trigger a hypersensitive response that rapidly kills cells near the infection site, depriving the pathogen of nutrients and preventing its spread. In this study, a diverse set of processes that link ipt activation to necrotic lesion formation were investigated in order to evaluate the potential of cytokinins as signals and/or mediators in plant defence against pathogens.
methodsThe binary pOp-ipt/LhGR system for dexamethasone-inducible ipt expression was used to increase endogenous cytokinin levels in transgenic tobacco. Changes in the levels of cytokinins and the stress hormones salicylic, jasmonic and abscisic acid following ipt activation were determined by ultra-performance liquid chromatography-electrospray tandem mass spectrometry (UPLC-MS/MS). Trends in hydrogen peroxide content and lipid peroxidation were monitored using the potassium iodide and malondialdehyde assays. The subcellular distribution of hydrogen peroxide was investigated using 3,3'-diaminobenzidine staining. The dynamics of transcripts related to photosynthesis and pathogen response were analysed by reverse transcription followed by quantitative PCR. The effects of cytokinins on photosynthesis were deciphered by analysing changes in chlorophyll fluorescence and leaf gas exchange. KEY
resultsPlants can produce sufficiently high levels of cytokinins to trigger fast cell death without any intervening chlorosis - a hallmark of the hypersensitive response. The results suggest that chloroplastic hydrogen peroxide orchestrates the molecular responses underpinning the hypersensitive-like response, including the inhibition of photosynthesis, elevated levels of stress hormones, oxidative membrane damage and stomatal closure.
conclusionsNecrotic lesion formation triggered by ipt activation closely resembles the hypersensitive response. Cytokinins may thus act as signals and/or mediators in plant defence against pathogen attack.
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