ArticleUltrasound in medicine & biology2013
Enhanced intracellular delivery of a model drug using microbubbles produced by a microfluidic device.
Article in Ultrasound in medicine & biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Targeted Microbubbles for Drug, Gene, and Cell Delivery in Therapy and Immunotherapy.Pharmaceutics · 2023Review
- Design of Improved Flow-Focusing Microchannel with Constricted Continuous Phase Inlet and Study of Fluid Flow Characteristics.Micromachines · 2022Article
- Microbubbles Stabilized by Protein Shell: From Pioneering Ultrasound Contrast Agents to Advanced Theranostic Systems.Pharmaceutics · 2022Review
- A Facile Single-Phase-Fluid-Driven Bubble Microfluidic Generator for Potential Detection of Viruses Suspended in Air.Biosensors · 2022Article
- Multiphase Microreactors Based on Liquid-Liquid and Gas-Liquid Dispersions Stabilized by Colloidal Catalytic Particles.Angewandte Chemie (International ed. in English) · 2022Review
- Microfluidic and Nanofluidic Intracellular Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis.Micromachines · 2021Review
- Integration of microbubbles with biomaterials in tissue engineering for pharmaceutical purposes.Heliyon · 2020Review
- Closed-loop feedback control of microbubble diameter from a flow-focusing microfluidic device.Biomicrofluidics · 2020Article
- Engineering the Echogenic Properties of Microfluidic Microbubbles Using Mixtures of Recombinant Protein and Amphiphilic Copolymers.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Efficacy of Sonothrombolysis Using Microbubbles Produced by a Catheter-Based Microfluidic Device in a Rat Model of Ischemic Stroke.Annals of biomedical engineering · 2019Article
- Control of Acoustic Cavitation for Efficient Sonoporation with Phase-Shift Nanoemulsions.Ultrasound in medicine & biology · 2019Article
- Engineering Theranostic Microbubbles Using Microfluidics for Ultrasound Imaging and Therapy: A Review.Ultrasound in medicine & biology · 2018Review
- Clinical study of ultrasound and microbubbles for enhancing chemotherapeutic sensitivity of malignant tumors in digestive system.Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2018Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- A flow focusing microfluidic device with an integrated Coulter particle counter for production, counting and size characterization of monodisperse microbubbles.Lab on a chip · 2018Article
- Enhanced bioconversion of hydrogen and carbon dioxide to methane using a micro-nano sparger system: mass balance and energy consumption.RSC advances · 2018Article
- Strategies for the enhanced intracellular delivery of nanomaterials.Drug discovery today · 2018Review
- In Vitro Sonothrombolysis Enhancement by Transiently Stable Microbubbles Produced by a Flow-Focusing Microfluidic Device.Annals of biomedical engineering · 2018Article
- Microfluidic manufacture of rt-PA -loaded echogenic liposomes.Biomedical microdevices · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Focal drug delivery to a vessel wall facilitated by intravascular ultrasound and microbubbles holds promise as a potential therapy for atherosclerosis. Conventional methods of microbubble administration result in rapid clearance from the bloodstream and significant drug loss. To address these limitations, we evaluated whether drug delivery could be achieved with transiently stable microbubbles produced in real time and in close proximity to the therapeutic site. Rat aortic smooth muscle cells were placed in a flow chamber designed to simulate physiological flow conditions. A flow-focusing microfluidic device produced 8 μm diameter monodisperse microbubbles within the flow chamber, and ultrasound was applied to enhance uptake of a surrogate drug (calcein). Acoustic pressures up to 300 kPa and flow rates up to 18 mL/s were investigated. Microbubbles generated by the flow-focusing microfluidic device were stabilized with a polyethylene glycol-40 stearate shell and had either a perfluorobutane (PFB) or nitrogen gas core. The gas core composition affected stability, with PFB and nitrogen microbubbles exhibiting half-lives of 40.7 and 18.2 s, respectively. Calcein uptake was observed at lower acoustic pressures with nitrogen microbubbles (100 kPa) than with PFB microbubbles (200 kPa) (p < 0.05, n > 3). In addition, delivery was observed at all flow rates, with maximal delivery (>70% of cells) occurring at a flow rate of 9 mL/s. These results demonstrate the potential of transiently stable microbubbles produced in real time and in close proximity to the intended therapeutic site for enhancing localized drug delivery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.