ReviewCurrent opinion in structural biology2013
Protein production from the structural genomics perspective: achievements and future needs.
Review in Current opinion in structural biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Towards routine accurate electron-density studies of biological macromolecules.Acta crystallographica. Section D, Structural biology · 2026Article
- Advancing the Research and Development of Enzyme Replacement Therapies for Lysosomal Storage Diseases.GEN biotechnology · 2022Article
- High-throughput cell-free screening of eukaryotic membrane protein expression in lipidic mimetics.Protein science : a publication of the Protein Society · 2022Article
- Fine Sampling of Sequence Space for Membrane Protein Structural Biology.Journal of molecular biology · 2021Article
- Construction of gateway-compatible baculovirus expression vectors for high-throughput protein expression and in vivo microcrystal screening.Scientific reports · 2020Article
- A multi-column plate adapter provides an economical and versatile high-throughput protein purification system.Protein expression and purification · 2018Article
- The N-terminal domain of Schmallenberg virus envelope protein Gc is highly immunogenic and can provide protection from infection.Scientific reports · 2017Article
- Protein purification and crystallization artifacts: The tale usually not told.Protein science : a publication of the Protein Society · 2016Article
- The impact of structural genomics: the first quindecennial.Journal of structural and functional genomics · 2016Review
- The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics.Advances in experimental medicine and biology · 2016Review
- Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells.Journal of molecular biology · 2015Article
- Baculovirus expression: old dog, new tricks.Bioengineered · 2015Article
- X-ray crystallography over the past decade for novel drug discovery - where are we heading next?Expert opinion on drug discovery · 2015Review
- Considerations for combined immune checkpoint modulation and radiation treatment.Radiation research · 2014Review
- Trends in structural coverage of the protein universe and the impact of the Protein Structure Initiative.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Recent advances in mammalian protein production.FEBS letters · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Despite a multitude of recent technical breakthroughs speeding high-resolution structural analysis of biological macromolecules, production of sufficient quantities of well-behaved, active protein continues to represent the rate-limiting step in many structure determination efforts. These challenges are only amplified when considered in the context of ongoing structural genomics efforts, which are now contending with multi-domain eukaryotic proteins, secreted proteins, and ever-larger macromolecular assemblies. Exciting new developments in eukaryotic expression platforms, including insect and mammalian-based systems, promise enhanced opportunities for structural approaches to some of the most important biological problems. Development and implementation of automated eukaryotic expression techniques promises to significantly improve production of materials for structural, functional, and biomedical research applications.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.