ArticleACS chemical neuroscience2013
Analysis of glutamate, GABA, noradrenaline, dopamine, serotonin, and metabolites using microbore UHPLC with electrochemical detection.
Article in ACS chemical neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Development of a high-throughput dual-stream liquid chromatography-tandem mass spectrometry method to screen for inhibitors of glutamate carboxypeptidase II.Rapid communications in mass spectrometry : RCM · 2025Article
- Advancements in Brain Research: The In Vivo/In Vitro Electrochemical Detection of Neurochemicals.Biosensors · 2024Review
- The High-Precision Liquid Chromatography with Electrochemical Detection (HPLC-ECD) for Monoamines Neurotransmitters and Their Metabolites: A Review.Molecules (Basel, Switzerland) · 2024Review
- Techniques for Measurement of Serotonin: Implications in Neuropsychiatric Disorders and Advances in Absolute Value Recording Methods.ACS chemical neuroscience · 2023Review
- Neuroprotective Effect of Nosustrophine in a 3xTg Mouse Model of Alzheimer's Disease.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Nosustrophine: An Epinutraceutical Bioproduct with Effects on DNA Methylation, Histone Acetylation and Sirtuin Expression in Alzheimer's Disease.Pharmaceutics · 2022Article
- Brain-Implantable Multifunctional Probe for Simultaneous Detection of Glutamate and GABA Neurotransmitters: Optimization and In Vivo Studies.Micromachines · 2022Article
- Letting the little light of mind shine: Advances and future directions in neurochemical detection.Neuroscience research · 2022Review
- Fabrication of Highly Sensitive Pt-black Electrochemical Sensors for GABA Detection.Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference · 2021Article
- Brain-Implantable Multifunctional Probe for Simultaneous Detection of Glutamate and GABA Neurotransmitters.Sensors and actuators. B, Chemical · 2021Article
- Time of Day-Dependent Alterations in Hippocampal Kynurenic Acid, Glutamate, and GABA in Adult Rats Exposed to Elevated Kynurenic Acid During Neurodevelopment.Frontiers in psychiatry · 2021Article
- Altered monoaminergic levels, spasticity, and balance disability following repetitive blast-induced traumatic brain injury in rats.Brain research · 2020Article
- Electrochemical measurement of serotonin by Au-CNT electrodes fabricated on microporous cell culture membranes.Microsystems & nanoengineering · 2020Article
- 3D-Printed electrochemical sensor-integrated transwell systems.Microsystems & nanoengineering · 2020Article
- Frontiers in Electrochemical Sensors for Neurotransmitter Detection: Towards Measuring Neurotransmitters as Chemical Diagnostics for Brain Disorders.Analytical methods : advancing methods and applications · 2019Article
- Analysis of neurotransmitter levels in addiction-related brain regions during synthetic cathinone self-administration in male Sprague-Dawley rats.Psychopharmacology · 2019Article
- GABA Detection with Nano-ITIES Pipet Electrode: A New Mechanism, Water/DCE-Octanoic Acid Interface.Analytical chemistry · 2018Article
- A Novel Microbiosensor Microarray for ContinuousFrontiers in neuroscience · 2018Article
- Synthesis and Biological Evaluation of Novel Multi-target-Directed Benzazepines Against Excitotoxicity.Molecular neurobiology · 2017Article
- Dexamethasone Alters the Appetite Regulation via Induction of Hypothalamic Insulin Resistance in Rat Brain.Molecular neurobiology · 2017Article
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4 authors.
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Abstract
The applicability of microbore ultrahigh performance liquid chromatography (UHPLC) with electrochemical detection for offline analysis of a number of well-known neurotransmitters in less than 10 μL microdialysis fractions is described. Two methods are presented for the analysis of monoamine or amino acid neurotransmitters, using the same UHPLC instrument. Speed of analysis of noradrenaline (NA), dopamine (DA), serotonin (5-HT), and the metabolites homovanillic acid (HVA), 5-hydroxyindole aceticacid (5-HIAA), and 3,4-dihydroxyphenylacetic acid (DOPAC) was predominated by the retention behavior of NA, the nonideal behavior of matrix components, and the loss in signal of 5-HT. This method was optimized to meet the requirements for detection sensitivity and minimizing the size of collected fractions, which determines temporal resolution in microdialysis. The amino acid neurotransmitters glutamate (Glu) and γ-aminobutyric acid (GABA) were analyzed after an automated derivatization procedure. Under optimized conditions, Glu was resolved from a number of early eluting system peaks, while the total runtime was decreased to 15 min by a 4-fold increase of the flow rate under UHPLC conditions. The detection limit for Glu and GABA was 10 nmol/L (15 fmol in 1.5 μL); the monoamine neurotransmitters had a detection limit between 32 and 83 pmol/L (0.16-0.42 fmol in 5 μL) in standard solutions. Using UHPLC, the analysis times varied from 15 min to less than 2 min depending on the complexity of the samples and the substances to be analyzed.
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