ArticleBioconjugate chemistry2013
Imaging cell surface glycosylation in vivo using "double click" chemistry.
Article in Bioconjugate chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 71 citations in OpenAlex.
- Double-Click Strategy Combining CuAAC and (Thia-) Diels-Alder Reactions; Application Toward Peptide Labeling.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Bio-orthogonal Glycan Imaging of Cultured Cells and Whole AnimalACS central science · 2025Article
- In Situ Glycan Analysis and Editing in Living Systems.JACS Au · 2024Review
- Metabolic Glycan Labeling of Cancer Cells Using Variably Acetylated Monosaccharides.Bioconjugate chemistry · 2022Article
- T-cell surface generation of dual bivalent, bispecific T-cell engaging, RNA duplex cross-linked antibodies (dbBiTERs) for re-directed tumor cell lysis.Biotechnology journal · 2022Article
- Tetrazine bioorthogonal chemistry derivedFrontiers in molecular biosciences · 2022Review
- Article
- Click Chemistry as a Tool for Cell Engineering and Drug Delivery.Molecules (Basel, Switzerland) · 2019Review
- Article
- CYP450 Enzymes Effect Oxygen-Dependent Reduction of Azide-Based Fluorogenic Dyes.ACS central science · 2017Article
- Controlled Detachment of Chemically Glued Cells.Bioconjugate chemistry · 2016Article
- Imaging Tumor Metabolism to Assess Disease Progression and Treatment Response.Clinical cancer research : an official journal of the American Association for Cancer Research · 2016Review
- Designing sugar mimetics: non-natural pyranosides as innovative chemical tools.Current opinion in chemical biology · 2016Review
- Imaging Glycosylation In Vivo by Metabolic Labeling and Magnetic Resonance Imaging.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2016Article
- Imaging Glycosylation In Vivo by Metabolic Labeling and Magnetic Resonance Imaging.Angewandte Chemie (International ed. in English) · 2016Article
- Bioorthogonal Click Chemistry-Based Synthetic Cell Glue.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Systemic Fluorescence Imaging of Zebrafish Glycans with Bioorthogonal Chemistry.Angewandte Chemie (International ed. in English) · 2015Article
- Site-Specific Glycoconjugation of Protein via Bioorthogonal Tetrazine Cycloaddition with a Genetically Encoded trans-Cyclooctene or Bicyclononyne.Bioconjugate chemistry · 2015Article
- Design strategies for bioorthogonal smart probes.Organic & biomolecular chemistry · 2014Review
- P53 and cancer-associated sialylated glycans are surrogate markers of cancerization of the bladder associated with Schistosoma haematobium infection.PLoS neglected tropical diseases · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Dynamic alterations in cell surface glycosylation occur in numerous biological processes that involve cell-cell communication and cell migration. We report here imaging of cell surface glycosylation in live mice using double click chemistry. Cell surface glycans were metabolically labeled using peracetylated azido-labeled N-acetylgalactosamine and then reacted, in the first click reaction, with either a cyclooctyne, in a Huisgen [3 + 2] cycloaddition, or with a Staudinger phosphine, via Staudinger ligation. The second click reaction was a [4 + 2] inverse electron demand Diels-Alder reaction between a trans-cyclooctene and a tetrazine, where the latter reagent had been fluorescently labeled with a far-red fluorophore. After administration of the fluorescent tetrazine, the bifunctional cyclooctyne-cyclooctene produced significant azido sugar-dependent fluorescence labeling of tumor, kidney, liver, spleen, and small intestine in vivo, where the kidney and tumor could be imaged noninvasively in the live mouse.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.