ArticleBMC developmental biology2013
Foamy virus for efficient gene transfer in regeneration studies.
Article in BMC developmental biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Optimized toolkit for the manipulation of immortalized axolotl fibroblasts.Methods (San Diego, Calif.) · 2025Article
- Adeno-associated viruses for efficient gene expression in the axolotl nervous system.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Adeno-associated viral tools to trace neural development and connectivity across amphibians.Developmental cell · 2025Article
- Baculovirus Production and Infection in Axolotls.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Now that We Got There, What Next?Methods in molecular biology (Clifton, N.J.) · 2023Article
- Efficient production of inhibitor-free foamy virus glycoprotein-containing retroviral vectors by proteoglycan-deficient packaging cells.Molecular therapy. Methods & clinical development · 2022Article
- The amazing and anomalous axolotls as scientific models.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Gene and transgenics nomenclature for the laboratory axolotl-Ambystoma mexicanum.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- The Axolotl's journey to the modern molecular era.Current topics in developmental biology · 2022Article
- Salamander Insights Into Ageing and Rejuvenation.Frontiers in cell and developmental biology · 2021Article
- Advancements to the Axolotl Model for Regeneration and Aging.Gerontology · 2020Review
- Foamy Virus Vectors Transduce Visceral Organs and Hippocampal Structures following In Vivo Delivery to Neonatal Mice.Molecular therapy. Nucleic acids · 2018Article
- Non-model model organisms.BMC biology · 2017Article
- Article
- Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease.Development (Cambridge, England) · 2014Article
- Establishing an Efficient Electroporation-Based Method to Manipulate Target Gene Expression in the Axolotl Brain.Cell transplantationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMolecular studies of appendage regeneration have been hindered by the lack of a stable and efficient means of transferring exogenous genes. We therefore sought an efficient integrating virus system that could be used to study limb and tail regeneration in salamanders.
resultsWe show that replication-deficient foamy virus (FV) vectors efficiently transduce cells in two different regeneration models in cell culture and in vivo. Injection of EGFP-expressing FV but not lentivirus vector particles into regenerating limbs and tail resulted in widespread expression that persisted throughout regeneration and reamputation pointing to the utility of FV for analyzing adult phenotypes in non-mammalian models. Furthermore, tissue specific transgene expression is achieved using FV vectors during limb regeneration.
conclusionsFV vectors are efficient mean of transferring genes into axolotl limb/tail and infection persists throughout regeneration and reamputation. This is a nontoxic method of delivering genes into axolotls in vivo/ in vitro and can potentially be applied to other salamander species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.