ArticleTissue engineering. Part C, Methods2013
Visualizing osteogenesis in vivo within a cell-scaffold construct for bone tissue engineering using two-photon microscopy.
Article in Tissue engineering. Part C, Methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Characterization of Biocompatibility of Functional Bioinks for 3D Bioprinting.Bioengineering (Basel, Switzerland) · 2023Review
- Arthritis-associated osteoclastogenic macrophage, AtoM, as a key player in pathological bone erosion.Inflammation and regeneration · 2022Review
- Ultra-low binder content 3D printed calcium phosphate graphene scaffolds as resorbable, osteoinductive matrices that support bone formation in vivo.Scientific reports · 2022Article
- High-Resolution Imaging for the Analysis and Reconstruction of 3D Microenvironments for Regenerative Medicine: An Application-Focused Review.Bioengineering (Basel, Switzerland) · 2021Review
- Accuracy of digital model generated from CT data with metal artifact reduction algorithm.Scientific reports · 2021Article
- An overview of de novo bone generation in animal models.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Review
- Intravital imaging of orthotopic and ectopic bone.Inflammation and regeneration · 2020Review
- Human amniotic fluid stem cells attract osteoprogenitor cells in bone healing.Journal of cellular physiology · 2020Article
- Evaluation of an Engineered Hybrid Matrix for Bone Regeneration via Endochondral Ossification.Annals of biomedical engineering · 2020Article
- Cellular and collagen reference values of gingival and periodontal ligament tissues in rats: a pilot study.Histochemistry and cell biology · 2019Article
- Article
- Review
- Spatiotemporal Analyses of Osteogenesis and Angiogenesis via Intravital Imaging in Cranial Bone Defect Repair.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2015Article
- Bone tissue engineering with a collagen-hydroxyapatite scaffold and culture expanded bone marrow stromal cells.Journal of biomedical materials research. Part B, Applied biomaterials · 2015Article
- Support Immersion Endoscopy in Post-Extraction Alveolar Bone Chambers: A New Window for Microscopic Bone Imaging In Vivo.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors.
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Abstract
Tissue-engineering therapies have shown early success in the clinic, however, the cell-biomaterial interactions that result in successful outcomes are not yet well understood and are difficult to observe. Here we describe a method for visualizing bone formation within a tissue-engineered construct in vivo, at a single-cell resolution, and in situ in three dimensions using two-photon microscopy. First, two-photon microscopy and histological perspectives were spatially linked using fluorescent reporters for cells in the skeletal lineage. In the process, the tissue microenvironment that precedes a repair-focused study was described. The distribution and organization of type I collagen in the calvarial microenvironment was also described using its second harmonic signal. Second, this platform was used to observe in vivo, for the first time, host cells, donor cells, scaffold, and new bone formation within cell-seeded constructs in a bone defect. We examined constructs during bone repair 4 and 6 weeks after implantation. New bone formed on scaffolds, primarily by donor cells. Host cells formed a new periosteal layer that covered the implant. Scaffold resorption appeared to be site specific, where areas near the top were removed and deeper areas were completely embedded in new mineral. Visualizing the in vivo progression of the cell and scaffold microenvironment will contribute to our understanding of tissue-engineered regeneration and should lead to the development of more streamlined and therapeutically powerful approaches.
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Registered trials
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