ArticleThe journal of physical chemistry. A2013
Simple method to enhance the photostability of the fluorescence reporter R6G for prolonged single-molecule studies.
Article in The journal of physical chemistry. A, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed, 20 citations in OpenAlex.
- Improved Dye Survival in Expansion Microscopy through Stabilizer-Conjugated Linkers.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
For fluorescence-based single-molecule studies, photobleaching of the dye reporter often limits the time window over which individual molecules can be followed. As such, many strategies, for example, using a cocktail of chemical reagents, have been developed to decrease the rate of photobleaching. Herein, we introduce a new and highly effective method to enhance the photostability of one of the commonly used fluorescent dyes, rhodamine 6G (R6G). We show that micrometer-sized polydimethylsiloxane (PDMS) wells, when the PDMS surface is properly treated, not only provide a confined environment for single-molecule detection but can also significantly increase the survival time of individual R6G molecules before photobleaching. Moreover, our results suggest, consistent with several previous studies, that R6G photobleaching involves a radical state.
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Registered trials
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