ArticlePlant methods2013
An efficient flat-surface collar-free grafting method for Arabidopsis thaliana seedlings.
Article in Plant methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Short-day photoperiod enhances graft success by increasing auxin accumulation.Plant signaling & behavior · 2026Article
- High-Throughput Hypocotyl Grafting and Vascular Reconnection Assessment in Arabidopsis Seedlings.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Sorbitol promotes the graft healing process in pears.Horticulture research · 2025Article
- C4 plants respond to phosphate starvation differently than C3 plants.Plant physiology · 2025Article
- Integrative Analyses of Metabolome and Transcriptome Reveal Scion-Stock Asymmetry Reduction and Shift of Sugar Metabolism During Graft Junction Formation in Malus Domestica ('Hanfu') Homograft.International journal of molecular sciences · 2025Article
- Here comes the sun: integration of light, temperature, and auxin during herbaceous plant grafting.Planta · 2025Review
- Supplementary far-red light enhances the quality and root development of double-root-cutting grafted watermelon seedlings.Frontiers in plant science · 2025Article
- Optimization of cassava (MethodsX · 2024Article
- Advances in understanding the graft healing mechanism: a review of factors and regulatory pathways.Horticulture research · 2024Article
- An Integrated Analysis of Anatomical and Sugar Contents Identifies How Night Temperatures Regulate the Healing Process of Oriental Melon Grafted onto Pumpkin.Plants (Basel, Switzerland) · 2024Article
- Local phytochrome signalling limits root growth in light by repressing auxin biosynthesis.Journal of experimental botany · 2023Article
- Loss of OPT3 function decreases phloem copper levels and impairs crosstalk between copper and iron homeostasis and shoot-to-root signaling in Arabidopsis thaliana.The Plant cell · 2023Article
- Systemic control of plant regeneration and wound repair.The New phytologist · 2023Review
- Arabidopsis Micro-grafting to Study the Systemic Signaling of Nutrient Status.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Callose deposited at soybean sieve element inhibits long-distance transport of Soybean mosaic virus.AMB Express · 2022Article
- SHORT-ROOT Controls Cell Elongation in the Etiolated Arabidopsis Hypocotyl.Molecules and cells · 2022Article
- Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis.Developmental cell · 2022Article
- Mix-and-match: an improved, fast and accessible protocol for hypocotyl micrografting of Arabidopsis seedlings with systemic ACC responses as a case study.Plant methods · 2022Article
- High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues.Development (Cambridge, England) · 2022Article
- Comparisons of Anatomical Characteristics and Transcriptomic Differences between Heterografts and Homografts inPlants (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGrafting procedures are an excellent tool to study long range signalling processes within a plant. In the last decade, suitable flat-surface grafting procedures for young Arabidopsis seedlings using a collar to support the graft have been developed, allowing the study of long-range signals from a molecular perspective.
resultsIn the modification presented here, scion and stock are put together on the medium without supporting elements, while cotyledons are removed from the scion, resulting in increased grafting success that can reach up to 100%. At the same time, the protocol enables to process as many as 36 seedlings per hour, which combined with the high success percentage represents increased efficiency per time unit.
conclusionsGrowing cotyledons usually push the scion and the rootstock away in the absence of a supporting element. Removing them at the grafting step greatly improved success rate and reduced post-grafting manipulations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.