ArticlePrenatal diagnosis2013
Global gene expression analysis of amniotic fluid cell-free RNA from recipient twins with twin-twin transfusion syndrome.
Article in Prenatal diagnosis, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Amniotic fluid cell-free transcriptome: a glimpse into fetal development and placental cellular dynamics during normal pregnancy.BMC medical genomics · 2020Trial
- Identification of gestational-age-dependent cell-free fetal mRNA transcriptional changes in human amniotic fluid.Journal of human genetics · 2026Article
- Amniotic fluid extracellular vesicle and cell-free RNA profiling reveals fetal adaptations in twin-twin transfusion syndrome.American journal of obstetrics & gynecology MFM · 2025Article
- Fetal metabolic adaptations to cardiovascular stress in twin-twin transfusion syndrome.iScience · 2023Article
- RNA-Seq of amniotic fluid cell-free RNA: a discovery phase study of the pathophysiology of congenital cytomegalovirus infection.American journal of obstetrics and gynecology · 2022Article
- Fetal maturation revealed by amniotic fluid cell-free transcriptome in rhesus macaques.JCI insight · 2022Article
- Identification of a novel gene signature in second-trimester amniotic fluid for the prediction of preterm birth.Scientific reports · 2022Article
- Amniotic Fluid microRNA in Severe Twin-Twin Transfusion Syndrome Cardiomyopathy-Identification of Differences and Predicting Demise.Journal of cardiovascular development and disease · 2022Article
- Amniotic fluid microRNA profiles in twin-twin transfusion syndrome with and without severe recipient cardiomyopathy.American journal of obstetrics and gynecology · 2021Article
- The Amniotic Fluid Cell-Free Transcriptome Provides Novel Information about Fetal Development and Placental Cellular Dynamics.International journal of molecular sciences · 2021Review
- Global gene expression analysis of cell-free RNA in amniotic fluid from women destined to develop preeclampsia.Medicine · 2019Observational
- Amniotic fluid transcriptomics reflects novel disease mechanisms in fetuses with myelomeningocele.American journal of obstetrics and gynecology · 2017Article
- A comparison of sample collection methods for quantifying cell-free fetal neurodevelopment transcripts in amniotic fluid.BMC research notes · 2016Article
- Males are from Mars, and females are from Venus: sex-specific fetal brain gene expression signatures in a mouse model of maternal diet-induced obesity.American journal of obstetrics and gynecology · 2016Article
- Assessing the fetal effects of maternal obesity via transcriptomic analysis of cord blood: a prospective case-control study.BJOG : an international journal of obstetrics and gynaecology · 2016Article
- The pathway not taken: understanding 'omics data in the perinatal context.American journal of obstetrics and gynecology · 2015Article
- CSAX: Characterizing Systematic Anomalies in eXpression Data.Journal of computational biology : a journal of computational molecular cell biology · 2015Article
- The amniotic fluid transcriptome as a guide to understanding fetal disease.Cold Spring Harbor perspectives in medicine · 2015Review
- Comparative Transcriptome Analysis of Cell-Free Fetal RNA from Amniotic Fluid and RNA from Amniocytes in Uncomplicated Pregnancies.PloS one · 2015Article
- RNA-Seq and expression microarray highlight different aspects of the fetal amniotic fluid transcriptome.Prenatal diagnosis · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 6 institutions in 2 countries.
Funding
Abstract
objectiveThe objective of this study was to understand the biological pathways involved in twin-twin transfusion syndrome (TTTS) by performing global gene expression analysis of amniotic fluid (AF) cell-free RNA.
methodsA prospective whole transcriptome microarray study analyzing cell-free RNA in AF from TTTS recipient twins and singleton controls was carried out. Significantly differentially regulated genes in TTTS cases (N = 8) versus matched controls (N = 8) were identified and pathways analyses performed. Significant gene expression differences between stage II TTTS recipients (N = 5) and stage III TTTS recipients with abnormal Doppler measurements (N = 5) were also analyzed.
resultsAnalysis of paired data from TTTS cases and controls revealed differential expression of 801 genes, which were significantly enriched for neurological disease and cardiovascular system pathways. We also identified cardiovascular genes and pathways associated with the presence of critically abnormal Doppler measurements in stage III TTTS recipients.
conclusionsThis study provides the first transcriptome-wide data on the impact of TTTS on fetal development. Our results show that gene expression involving neurological and cardiovascular pathways are altered in recipient fetuses prior to surgical treatment. This has relevance for the origins of long-term complications seen in survivors and for the development of future fetal biomarkers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.