ArticleNature protocols2013
Constructing droplet interface bilayers from the contact of aqueous droplets in oil.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 154 citations in OpenAlex.
- Jammed interconnected bilayer emulsions as 3D-printable biological tissue mimics.Nature materials · 2026Article
- The Ion Permeability of DNA Nanotube Channels.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Structural basis for independent pore function of Vpb4 from Bacillus thuringiensis.Nature communications · 2026Article
- Triply Responsive Control of Ion Transport with an Artificial Channel Creates a Switchable AND to OR Logic Gate.Angewandte Chemie (International ed. in English) · 2026Article
- Towards a perfusion system for functional study of membrane proteins with independent control of the electrical and chemical transmembrane potential.Biophysical reviews · 2025Review
- Neuromorphic ionic computing in droplet interface synapses.Science advances · 2025Article
- Hybrid Exb/Mot stators require substitutions distant from the chimeric pore to power flagellar rotation.Journal of bacteriology · 2024Article
- Ion transport and ultra-efficient osmotic power generation in boron nitride nanotube porins.Science advances · 2024Article
- Molecular transport enhancement in pure metallic carbon nanotube porins.Nature materials · 2024Article
- High-throughput single biomarker identification using droplet nanopore.Chemical science · 2024Article
- Manipulation of encapsulated artificial phospholipid membranes using sub-micellar lysolipid concentrations.Communications chemistry · 2024Article
- New insights into the structure and dynamics of the TOM complex in mitochondria.Biochemical Society transactions · 2024Review
- Tracking the Activity and Position of Mitochondrial β-Barrel Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Transmembrane coupling of liquid-like protein condensates.Nature communications · 2023Article
- Single-molecule tracking of perfringolysin O assembly and membrane insertion uncoupling.The FEBS journal · 2023Article
- Domain Size Regulation in Phospholipid Model Membranes Using Oil Molecules and Hybrid Lipids.The journal of physical chemistry. B · 2022Article
- Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation.Nature communications · 2022Article
- Encapsulated droplet interface bilayers as a platform for high-throughput membrane studies.Soft matter · 2022Article
- Reconstitution of Membrane-tethered Minimal Actin Cortices on Supported Lipid Bilayers.Journal of visualized experiments : JoVE · 2022Article
- Molecular substructure of the liquid-ordered phase formed by sphingomyelin and cholesterol: sphingomyelin clusters forming nano-subdomains are a characteristic feature.Biophysical reviews · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
We describe a protocol for forming an artificial lipid bilayer by contacting nanoliter aqueous droplets in an oil solution in the presence of phospholipids. A lipid monolayer forms at each oil-water interface, and when two such monolayers touch, a bilayer is created. Droplet interface bilayers (DIBs) are a simple way to generate stable bilayers suitable for single-channel electrophysiology and optical imaging from a wide variety of preparations, ranging from purified proteins to reconstituted eukaryotic cell membrane fragments. Examples include purified proteins from the α-hemolysin pore from Staphylococcus aureus, the anthrax toxin pore and the 1.2-MDa mouse mechanosensitive channel MmPiezo1. Ion channels and ionotropic receptors can also be reconstituted from membrane fragments without further purification. We describe two approaches for forming DIBs. In one approach, a lipid bilayer is created between two aqueous droplets submerged in oil. In the other approach, a membrane is formed between an aqueous droplet and an agarose hydrogel, which allows imaging in addition to electrical recordings. The protocol takes <30 min, including droplet generation, monolayer assembly and bilayer formation. In addition to the main protocol, we also describe the preparation of Ag/AgCl electrodes and sample preparation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.