ArticleJournal of biomedical optics2013
Photoacoustic lifetime contrast between methylene blue monomers and self-quenched dimers as a model for dual-labeled activatable probes.
Article in Journal of biomedical optics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Optimization of light source parameters for photoacoustic imaging: trade-offs, technologies, and clinical considerations.JPhys photonics · 2026Review
- Electrochemical Oxidation Degradation of Methylene Blue Dye on 3D-Printed Anode Electrodes.Polymers · 2025Article
- Acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probes.Nature communications · 2025Article
- Effect of Hyaluronic Acid on the Activity of Methylene Blue in Photogeneration ofMolecules (Basel, Switzerland) · 2024Article
- Intermolecular interactions in mixed dye systems and the effects on dye wastewater treatment processes.RSC advances · 2024Article
- Non-invasive evaluation of transdermal drug delivery using 3-D transient triplet differential (TTD) photoacoustic imaging.Photoacoustics · 2023Article
- A red-green photochromic bacterial protein as a new contrast agent for improved photoacoustic imaging.Photoacoustics · 2022Article
- Design Principles Governing the Development of Theranostic Anticancer Agents and Their Nanoformulations with Photoacoustic Properties.Pharmaceutics · 2022Review
- Recent Progress Toward Imaging Application of Multifunction Sonosensitizers in Sonodynamic Therapy.International journal of nanomedicine · 2022Review
- Activatable Carbocyanine Dimers for Photoacoustic and Fluorescent Detection of Protease Activity.ACS sensors · 2021Article
- Methylene blue-filled biodegradable polymer particles as a contrast agent for optical coherence tomography.Biomedical optics express · 2020Article
- A Conformationally Restricted Aza-BODIPY Platform for Stimulus-Responsive Probes with Enhanced Photoacoustic Properties.Journal of the American Chemical Society · 2019Article
- Development of a blood oxygenation phantom for photoacoustic tomography combined with online pO2 detection and flow spectrometry.Journal of biomedical optics · 2019Article
- Listening to tissues with new light: recent technological advances in photoacoustic imaging.Journal of optics (2010) · 2019Article
- Strategies for Image-Guided Therapy, Surgery, and Drug Delivery Using Photoacoustic Imaging.Theranostics · 2019Review
- Cell penetrating peptide-modified nanoparticles for tumor targeted imaging and synergistic effect of sonodynamic/HIFU therapy.International journal of nanomedicine · 2019Article
- A Mechanistic Investigation of Methylene Blue and Heparin Interactions and Their Photoacoustic Enhancement.Bioconjugate chemistry · 2018Article
- Molecular Photoacoustic Contrast Agents: Design Principles & Applications.Photochemistry and photobiology · 2018Review
- Small Molecule Optoacoustic Contrast Agents: An Unexplored Avenue for Enhancing In Vivo Imaging.Molecules (Basel, Switzerland) · 2018Review
- Recent progress in photoacoustic molecular imaging.Current opinion in chemical biology · 2018Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Activatable photoacoustic probes efficiently combine the high spatial resolution and penetration depth of ultrasound with the high optical contrast and versatility of molecular imaging agents. Our approach is based on photoacoustic probing of the excited-state lifetime of methylene blue (MB), a fluorophore widely used in clinical therapeutic and diagnostic applications. Upon aggregation, static quenching between the bound molecules dramatically shortens their lifetime by three orders of magnitude. We present preliminary results demonstrating the ability of photoacoustic imaging to probe the lifetime contrast between monomers and dimers with high sensitivity in cylindrical phantoms. Gradual dimerization enhancement, driven by the addition of increasing concentrations of sodium sulfate to a MB solution, showed that lifetime-based photoacoustic probing decreases linearly with monomer concentration. Similarly, the addition of 4 mM sodium dodecyl sulfate, a concentration that amplifies MB aggregation and reduces the monomer concentration by more than 20-fold, led to a signal decrease of more than 20 dB compared to a solution free of surfactant. These results suggest that photoacoustic imaging can be used to selectively detect the presence of monomers. We conclude by discussing the implementation of the monomer-dimer contrast mechanism for the development of an enzyme-specific activatable probe.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.