ArticleIranian journal of basic medical sciences2013
The effect of the crocus sativus L. Carotenoid, crocin, on the polymerization of microtubules, in vitro.
Article in Iranian journal of basic medical sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- A comprehensive systematic review of human trials investigating herbal treatments for Alzheimer's disease and dementia.Acta neuropsychiatrica · 2026Review
- Recent advancements in the role of phytochemicals and medicinal plants in prophylaxis and management of Alzheimer's disease.Iranian journal of basic medical sciences · 2024Review
- Review
- Current Knowledge of the Antidepressant Activity of Chemical Compounds fromPharmaceuticals (Basel, Switzerland) · 2022Review
- Possible protective effects of crocin on destructive side effects of cyclo-phosphamide in mice ovarian tissue: Evaluation of histomorphometrical and biochemical changes.Veterinary research forum : an international quarterly journal · 2021Article
- Saffron extract attenuates neuroinflammation in rmTBI mouse model by suppressing NLRP3 inflammasome activation via SIRT1.PloS one · 2021Article
- Role of crocin in several cancer cell lines: An updated review.Iranian journal of basic medical sciences · 2020Review
- TheFrontiers in neuroscience · 2019Article
- Antioxidant Properties of Crocus Sativus L. and Its Constituents and Relevance to Neurodegenerative Diseases; Focus on Alzheimer's and Parkinson's Disease.Current neuropharmacology · 2019Review
- The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype.Iranian journal of basic medical sciences · 2018Article
- Antiproliferative Activity of Crocin Involves Targeting of Microtubules in Breast Cancer Cells.Scientific reports · 2017Article
- Possible Role of Common Spices as a Preventive and Therapeutic Agent for Alzheimer's Disease.International journal of preventive medicine · 2017Review
- Neuroprotective effects of crocin on the histopathological alterations following brain ischemia-reperfusion injury in rat.Iranian journal of basic medical sciences · 2014Article
- Crocin enhanced functional recovery after sciatic nerve crush injury in rats.Iranian journal of basic medical sciences · 2013Article
- Crocin improved learning and memory impairments in streptozotocin-induced diabetic rats.Iranian journal of basic medical sciences · 2013Article
- Effects of Crocin Supplementation during In Vitro Maturation of Mouse Oocytes on Glutathione Synthesis and Cytoplasmic Maturation.International journal of fertility & sterilityArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
OBJECTIVE(S): Crocin, as the main carotenoid of saffron, has shown anti-tumor activity both in vitro and in vivo. Crocin might interact with cellular proteins and modulate their functions, but the exact target of this carotenoid and the other compounds of the saffron have not been discovered yet. Microtubular proteins, as one of the most important proteins inside the cells, have several functions in nearly all kinds of cellular processes. The aim of this study was to investigate whether crocin affects microtubule polymerization and tubulin structure. MATERIALS AND
methodsMicrotubules were extracted from sheep brains after two cycles of temperature-dependant assembly-disassembly in the polymerization buffer (PMG). Then phosphocellulose P11 column was used to prepare MAP-free tubulin. Turbidimetric assay of microtubules was performed by incubation of tubulins at 37 ºC in PIPES buffer. To investigate the intrinsic fluorescence spectra of tubulins, the emission spectra of tryptophans was monitored. To test the interaction of crocin with tubulin in more details, ANS has been used.
resultsCrocin extremely affected the tubulin polymerization and structure. Ultraviolet spectroscopy indicated that crocin increased polymerization of microtubules by nearly a factor of two. Fluorescence spectroscopic data also pointed to significant conformational changes of tubulin.
conclusionWe showed that crocin increased tubulin polymerization and microtubule nucleation rate and this effect was concentration dependant. After entering cell, crocin can modulate cellular proteins and their functions. Concerning the results of this study, crocin would be able to affect several cell processes through interaction with tubulin proteins or microtubules.
Indexed as
Identifiers
23638298PMC3637910W2132476442What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.