ArticlePLoS neglected tropical diseases2013
Second international diagnostic accuracy study for the serological detection of West Nile virus infection.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A global database of West Nile virus host prevalence and competence.Scientific data · 2026Article
- Seroprevalence of West Nile Virus in Blood Donors in Mainland Portugal.Tropical medicine and infectious disease · 2025Article
- A 20-year historical review of West Nile virus since its initial emergence in North America: Has West Nile virus become a neglected tropical disease?PLoS neglected tropical diseases · 2021Review
- Evaluation of West Nile Virus Diagnostic Capacities in Veterinary Laboratories of the Mediterranean and Black Sea Regions.Pathogens (Basel, Switzerland) · 2020Article
- Serological Diagnosis of Flavivirus-Associated Human Infections.Diagnostics (Basel, Switzerland) · 2020Review
- Review
- Specific detection of dengue and Zika virus antibodies using envelope proteins with mutations in the conserved fusion loop.Emerging microbes & infections · 2017Article
- Improved reliability of serological tools for the diagnosis of West Nile fever in horses within Europe.PLoS neglected tropical diseases · 2017Article
- West Nile Virus in Europe and Safety of Blood Transfusion.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2016Review
- Current concepts in the prevention of pathogen transmission via blood/plasma-derived products for bleeding disorders.Blood reviews · 2016Review
- Distinguishing West Nile virus infection using a recombinant envelope protein with mutations in the conserved fusion-loop.BMC infectious diseases · 2014Article
- Spatial and temporal distribution of West Nile virus in horses in Israel (1997-2013)--from endemic to epidemics.PloS one · 2014Article
- Review
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Authors and funding
6 authors.
Funding
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Abstract
backgroundIn recent decades, sporadic cases and outbreaks in humans of West Nile virus (WNV) infection have increased. Serological diagnosis of WNV infection can be performed by enzyme-linked immunosorbent assay (ELISA), immunofluorescence assay (IFA) neutralization test (NT) and by hemagglutination-inhibition assay. The aim of this study is to collect updated information regarding the performance accuracy of WNV serological diagnostics. METHODOLOGY/PRINCIPAL
findingsIn 2011, the European Network for the Diagnostics of Imported Viral Diseases-Collaborative Laboratory Response Network (ENIVD-CLRN) organized the second external quality assurance (EQA) study for the serological diagnosis of WNV infection. A serum panel of 13 samples (included sera reactive against WNV, plus specificity and negative controls) was sent to 48 laboratories involved in WNV diagnostics. Forty-seven of 48 laboratories from 30 countries participated in the study. Eight laboratories achieved 100% of concurrent and correct results. The main obstacle in other laboratories to achieving similar performances was the cross-reactivity of antibodies amongst heterologous flaviviruses. No differences were observed in performances of in-house and commercial test used by the laboratories. IFA was significantly more specific compared to ELISA in detecting IgG antibodies. The overall analytical sensitivity and specificity of diagnostic tests for IgM detection were 50% and 95%, respectively. In comparison, the overall sensitivity and specificity of diagnostic tests for IgG detection were 86% and 69%, respectively. CONCLUSIONS/SIGNIFICANCE: This EQA study demonstrates that there is still need to improve serological tests for WNV diagnosis. The low sensitivity of IgM detection suggests that there is a risk of overlooking WNV acute infections, whereas the low specificity for IgG detection demonstrates a high level of cross-reactivity with heterologous flaviviruses.
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