ArticlePloS one2013
Identification and analysis of multi-protein complexes in placenta.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- DNase Activities of a Highly Stable High-Molecular-Mass Multiprotein Complex Isolated from Different Organs of the Sea CucumberInternational journal of molecular sciences · 2026Article
- Oxidoreductase Activity of a Stable Multi-protein Complex from the Sea Cucumber Eupentacta fraudatrix.The protein journal · 2026Article
- Phosphatase Activities of a Highly Stable High-Molecular-Mass Multiprotein Complex Isolated from Different Organs of the Sea CucumberInternational journal of molecular sciences · 2026Article
- Stable and Specific Multiprotein Complexes with High Molecular Masses in Different Organs of the Sea CucumberInternational journal of molecular sciences · 2026Article
- Very Stable High-Molecular-Mass Multiprotein Complexes in Different Organs of the Sea CucumberMolecules (Basel, Switzerland) · 2025Article
- Exploring the origins of neurodevelopmental proteasomopathies associated with cardiac malformations: are neural crest cells central to certain pathological mechanisms?Frontiers in cell and developmental biology · 2024Article
- Blue Native PAGE-Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes: Detection of a Cerebellar α1/α6-Subunits Containing γ-Aminobutyric Acid Type A Receptor Subtype.International journal of molecular sciences · 2023Article
- Two-Dimensional Gel Electrophoresis and 2D-DIGE.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Proteomic Approaches in the Study of Placenta of Pregnancy Complicated by Gestational Diabetes Mellitus.Biomedicines · 2022Review
- Protease and DNase Activities of a Very Stable High-Molecular-Mass Multiprotein Complex from Sea CucumberInternational journal of molecular sciences · 2022Article
- Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi.Current issues in molecular biology · 2022Article
- Very Stable Two Mega Dalton High-Molecular-Mass Multiprotein Complex from Sea CucumberMolecules (Basel, Switzerland) · 2021Article
- Plasma membrane proteomic analysis of human Gastric Cancer tissues: revealing flotillin 1 as a marker for Gastric Cancer.BMC cancer · 2015Article
- Basics and recent advances of two dimensional- polyacrylamide gel electrophoresis.Clinical proteomics · 2014Review
- Extremely stable soluble high molecular mass multi-protein complex with DNase activity in human placental tissue.PloS one · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Placental malfunction induces pregnancy disorders which contribute to life-threatening complications for both the mother and the fetus. Identification and characterization of placental multi-protein complexes is an important step to integratedly understand the protein-protein interaction networks in placenta which determine placental function. In this study, blue native/sodium dodecyl sulfate polyacrylamide gel electrophoresis (BN/SDS-PAGE) and Liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to screen the multi-protein complexes in placenta. 733 unique proteins and 34 known and novel heterooligomeric multi-protein complexes including mitochondrial respiratory chain complexes, integrin complexes, proteasome complexes, histone complex, and heat shock protein complexes were identified. A novel protein complex, which involves clathrin and small conductance calcium-activated potassium (SK) channel protein 2, was identified and validated by antibody based gel shift assay, co-immunoprecipitation and immunofluorescence staining. These results suggest that BN/SDS-PAGE, when integrated with LC-MS/MS, is a very powerful and versatile tool for the investigation of placental protein complexes. This work paves the way for deeper functional characterization of the placental protein complexes associated with pregnancy disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.