ArticlePloS one2013
Cholesterol depletion disorganizes oocyte membrane rafts altering mouse fertilization.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Impact of Breast Cancer on Ovarian Function: Dysregulation of Cholesterol Homeostasis in Cumulus Cells and Follicular Fluid.Cancers · 2026Article
- Abnormal cholesterol-cholesteryl ester metabolism impairs mouse oocyte quality during ovarian aging.Cellular & molecular biology letters · 2025Article
- Characterization of the Lipid Profile of Immature and In Vitro Matured Cat Oocytes.Molecular reproduction and development · 2025Article
- Biomembrane structure at the molecular level and its application in precision medicine.Biophysics reviews · 2025Review
- Folic acid supplementation alleviates donor cattle's heat stress and improves in vitro embryo production efficiency in summer.Journal of animal science · 2025Article
- Optimization of bovine oocyte cryopreservation: membrane fusion competence and cell death of linoleic acid-Frontiers in veterinary science · 2025Article
- Association between depression and infertility risk among American women aged 18-45 years: the mediating effect of the NHHR.Lipids in health and disease · 2024Article
- Maternal hypercholesterolemia would increase the incidence of embryo aneuploidy in couples with recurrent implantation failure.European journal of medical research · 2023Article
- Distribution of tetraspanins in bovine ovarian tissue and fresh/vitrified oocytes.Histochemistry and cell biology · 2023Article
- Article
- Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares.Frontiers in cell and developmental biology · 2023Article
- Repurposing phenothiazines for cancer therapy: compromising membrane integrity in cancer cells.Frontiers in oncology · 2023Review
- Improvement of Fertilization Capacity and Developmental Ability of Vitrified Bovine Oocytes by JUNO mRNA Microinjection and Cholesterol-Loaded Methyl-β-Cyclodextrin Treatment.International journal of molecular sciences · 2022Article
- Seasonal Variations in the Lipid Profile of the Ovarian Follicle in Italian Mediterranean Buffaloes.Animals : an open access journal from MDPI · 2022Article
- Mammalian cumulus-oocyte complex communication: a dialog through long and short distance messaging.Journal of assisted reproduction and genetics · 2022Review
- Implications of High-Density Cholesterol Metabolism for Oocyte Biology and Female Fertility.Frontiers in cell and developmental biology · 2022Review
- Cryo-EM structure of the B cell co-receptor CD19 bound to the tetraspanin CD81.Science (New York, N.Y.) · 2021Article
- Lipid composition of the cancer cell membrane.Journal of bioenergetics and biomembranes · 2020Review
- Follicular extracellular vesicles enhance meiotic resumption of domestic cat vitrified oocytes.Scientific reports · 2020Article
- Compensatory endocytosis occurs after cortical granule exocytosis in mouse eggs.Journal of cellular physiology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drastic membrane reorganization occurs when mammalian sperm binds to and fuses with the oocyte membrane. Two oocyte protein families are essential for fertilization, tetraspanins and glycosylphosphatidylinositol-anchored proteins. The firsts are associated to tetraspanin-enriched microdomains and the seconds to lipid rafts. Here we report membrane raft involvement in mouse fertilization assessed by cholesterol modulation using methyl-β-cyclodextrin. Cholesterol removal induced: (1) a decrease of the fertilization rate and index; and (2) a delay in the extrusion of the second polar body. Cholesterol repletion recovered the fertilization ability of cholesterol-depleted oocytes, indicating reversibility of these effects. In vivo time-lapse analyses using fluorescent cholesterol permitted to identify the time-point at which the probe is mainly located at the plasma membrane enabling the estimation of the extent of the cholesterol depletion. We confirmed that the mouse oocyte is rich in rafts according to the presence of the raft marker lipid, ganglioside GM1 on the membrane of living oocytes and we identified the coexistence of two types of microdomains, planar rafts and caveolae-like structures, by terms of two differential rafts markers, flotillin-2 and caveolin-1, respectively. Moreover, this is the first report that shows characteristic caveolae-like invaginations in the mouse oocyte identified by electron microscopy. Raft disruption by cholesterol depletion disturbed the subcellular localization of the signal molecule c-Src and the inhibition of Src kinase proteins prevented second polar body extrusion, consistent with a role of Src-related kinases in fertilization via signaling complexes. Our data highlight the functional importance of intact membrane rafts for mouse fertilization and its dependence on cholesterol.
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