ArticlePloS one2013
Reference gene selection for real-time quantitative PCR analysis of the mouse uterus in the peri-implantation period.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- Trial
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- Temporal dynamics of uterine immune microenvironment remodeling in a murine model of adenomyosis.Molecular human reproduction · 2025Article
- Comprehensive Genetic and Molecular Characterization Confirms Hepatic Stellate Cell Origin of the Immortal Col-GFP HSC Line.International journal of molecular sciences · 2025Article
- Engineering Baker's Yeast for Efficient cAMP Synthesis via Regulation of PKA Activity.Foods (Basel, Switzerland) · 2025Article
- Transcriptome profiling reveals dysregulation of inflammatory and protein synthesis genes in PCOS.Scientific reports · 2024Article
- Impaired fertility in adenomyosis: a murine model reveals endometrial receptivity and progesterone resistance imbalances.Reproduction (Cambridge, England) · 2024Article
- Ghrelin misbalance affects mice embryo implantation and pregnancy success by uterine immune dysregulation and nitrosative stress.Frontiers in endocrinology · 2023Article
- Development of severe colitis is associated with lung inflammation and pathology.Frontiers in immunology · 2023Article
- Granulocyte colony-stimulating factor is not pathogenic in lupus nephritis.Immunity, inflammation and disease · 2021Article
- Improved diabetic wound healing by LFcinB is associated with relevant changes in the skin immune response and microbiota.Molecular therapy. Methods & clinical development · 2021Article
- Article
- Validation of Reference Genes for Gene Expression Normalization in RAW264.7 Cells under Different Conditions.BioMed research international · 2019Article
- An immortalized steroidogenic goat granulosa cell line as a model system to study the effect of the endoplasmic reticulum (ER)-stress response on steroidogenesis.The Journal of reproduction and development · 2017Article
- Article
- Effects of whole genome duplication on cell size and gene expression in mouse embryonic stem cells.The Journal of reproduction and development · 2016Article
- The 60S ribosomal protein L13 is the most preferable reference gene to investigate gene expression in selected organs from turkeys and chickens, in context of different infection models.Veterinary research · 2016Article
- Expression and regulation of ATF6α in the mouse uterus during embryo implantation.Reproductive biology and endocrinology : RB&E · 2016Article
- Estrogen maintains myometrial tumors in a lymphangioleiomyomatosis model.Endocrine-related cancer · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of uterine gene expression patterns is valuable for understanding the biological and molecular mechanisms that occur during embryo implantation. Real-time quantitative RT-PCR (qRT-PCR) is an extremely sensitive technique that allows for the precise quantification of mRNA abundance; however, selecting stable reference genes suitable for the normalization of qRT-PCR data is required to avoid the misinterpretation of experimental results and erroneous analyses. This study employs several mouse models, including an early pregnancy, a pseudopregnancy, a delayed implantation and activation, an artificial decidualization and a hormonal treatment model; ten candidate reference genes (PPIA, RPLP0, HPRT1, GAPDH, ACTB, TBP, B2M, 18S, UBC and TUBA) that are found in uterine tissues were assessed for their suitability as internal controls for relative qRT-PCR quantification. GeNorm(PLUS), NormFinder, and BestKeeper were used to evaluate these candidate reference genes, and all of these methods identified RPLP0 and GAPDH as the most stable candidates and B2M and 18S as the least stable candidates. However, when the different models were analyzed separately, the reference genes exhibited some variation in their expression levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.