ArticlePloS one2013
Multiplex detection of plant pathogens using a microsphere immunoassay technology.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 78 citations in OpenAlex.
- Emerging technologies for in-field plant virus detection: innovations and future directions.The Journal of general virology · 2025Review
- Current status and future perspectives of the diagnostic of plant bacterial pathogens.Frontiers in plant science · 2025Review
- Development of peptide nucleic acid-based bead array technology for Bacillus cereus detection.Scientific reports · 2023Article
- Scope of Onsite, Portable Prevention Diagnostic Strategies forBiosensors · 2023Review
- Current and emerging trends in techniques for plant pathogen detection.Frontiers in plant science · 2023Review
- Rapid, Sensitive, and Selective Quantification ofMicroorganisms · 2022Article
- Nanotechnology for Nanophytopathogens: From Detection to the Management of Plant Viruses.BioMed research international · 2022Review
- Graphene-Based Sensor for Detection of Bacterial Pathogens.Sensors (Basel, Switzerland) · 2021Article
- Biosensor Technologies for Early Detection and Quantification of Plant Pathogens.Frontiers in chemistry · 2021Review
- Double antibody pairs sandwich-ELISA (DAPS-ELISA) detects Acidovorax citrulli serotypes with broad coverage.PloS one · 2020Article
- Advanced Diagnostic Approaches for Necrotrophic Fungal Pathogens of Temperate Legumes With a Focus onFrontiers in microbiology · 2019Review
- 2D transition metal dichalcogenides with glucan multivalency for antibody-free pathogen recognition.Nature communications · 2018Article
- Development of a sensitive Luminex xMAP-based microsphere immunoassay for specific detection of Iris yellow spot virus.Virology journal · 2018Article
- A rapid colorimetric immunoassay for the detection of pathogenic bacteria on poultry processing plants using cotton swabs and nanobeads.Mikrochimica acta · 2018Article
- Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection.Frontiers in plant science · 2017Review
- Simple and portable magnetic immunoassay for rapid detection and sensitive quantification of plant viruses.Applied and environmental microbiology · 2015Article
- Relationship between Expression of Cellular Receptor-27.8 kDa and Lymphocystis Disease Virus (LCDV) Infection.PloS one · 2015Article
- A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection.Lab on a chip · 2013Article
- Implementation of microfluidic sandwich ELISA for superior detection of plant pathogens.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant pathogens are a serious problem for seed export, plant disease control and plant quarantine. Rapid and accurate screening tests are urgently required to protect and prevent plant diseases spreading worldwide. A novel multiplex detection method was developed based on microsphere immunoassays to simultaneously detect four important plant pathogens: a fruit blotch bacterium Acidovorax avenae subsp. citrulli (Aac), chilli vein-banding mottle virus (CVbMV, potyvirus), watermelon silver mottle virus (WSMoV, tospovirus serogroup IV) and melon yellow spot virus (MYSV, tospovirus). An antibody for each plant pathogen was linked on a fluorescence-coded magnetic microsphere set which was used to capture corresponding pathogen. The presence of pathogens was detected by R-phycoerythrin (RPE)-labeled antibodies specific to the pathogens. The assay conditions were optimized by identifying appropriate antibody pairs, blocking buffer, concentration of RPE-labeled antibodies and assay time. Once conditions were optimized, the assay was able to detect all four plant pathogens precisely and accurately with substantially higher sensitivity than enzyme-linked immunosorbent assay (ELISA) when spiked in buffer and in healthy watermelon leaf extract. The assay time of the microsphere immunoassay (1 hour) was much shorter than that of ELISA (4 hours). This system was also shown to be capable of detecting the pathogens in naturally infected plant samples and is a major advancement in plant pathogen detection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.