ArticlePloS one2013
Role of LytF and AtlS in eDNA release by Streptococcus gordonii.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Genetic and functional characterization of the natural transformation system inMicrobiology (Reading, England) · 2026Article
- LytF contributes to pilus extrusion during natural competence inJournal of bacteriology · 2026Article
- LytF contributes to pilus extrusion during natural competence inbioRxiv : the preprint server for biology · 2025Article
- Understanding the Matrix: The Role of Extracellular DNA in Oral Biofilms.Frontiers in oral health · 2021Review
- Competence-Stimulating-Peptide-Dependent Localized Cell Death and Extracellular DNA Production in Streptococcus mutans Biofilms.Applied and environmental microbiology · 2020Article
- Harnessing the Potential of Killers and Altruists within the Microbial Community: A Possible Alternative to Antibiotic Therapy?Antibiotics (Basel, Switzerland) · 2019Review
- Gymnemic Acids Inhibit Adhesive Nanofibrillar MediatedFrontiers in microbiology · 2019Article
- Susceptibility patterns and the role of extracellular DNA in Staphylococcus epidermidis biofilm resistance to physico-chemical stress exposure.BMC microbiology · 2018Article
- Streptococcus gordonii comCDE (competence) operon modulates biofilm formation with Candida albicans.Microbiology (Reading, England) · 2015Article
- Article
- Two-component system VicRK regulates functions associated with establishment of Streptococcus sanguinis in biofilms.Infection and immunity · 2014Article
- The impact of horizontal gene transfer on the adaptive ability of the human oral microbiome.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
Extracellular DNA (eDNA) is an important component of the biofilm matrix produced by many bacteria. In general, the release of eDNA is associated with the activity of muralytic enzymes leading to obvious cell lysis. In the Gram-positive oral commensal Streptococcus gordonii, eDNA release is dependent on pyruvate oxidase generated hydrogen peroxide (H2O2). Addition of H2O2 to cells grown under conditions non-permissive for H2O2 production causes eDNA release. Furthermore, eDNA release is maximal under aerobic growth conditions known to induce pyruvate oxidase gene expression and H2O2 production. Obvious cell lysis, however, does not occur. Two enzymes have been recently associated with eDNA release in S. gordonii. The autolysin AtlS and the competence regulated murein hydrolase LytF. In the present report, we investigated the role of both proteins in the H2O2 dependent eDNA release process. Single and double mutants in the respective genes for LytF and AtlS released less eDNA under normal growth conditions, but the AtlS mutant was still inducible for eDNA release by external H2O2. Moreover, we showed that the AtlS mutation interfered with the ability of S. gordonii to produce eDNA release inducing amounts of H2O2. Our data support a role of LytF in the H2O2 eDNA dependent release of S. gordonii as part of the competence stress pathway responding to oxidative stress.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.