ArticlePloS one2013
Defining the subcellular interface of nanoparticles by live-cell imaging.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 65 citations in OpenAlex.
- Evidence of Copper Nanoparticles and Poly I:C Modulating Cas9 Interaction and Cleavage of COR (Conserved Omicron RNA).Bioengineering (Basel, Switzerland) · 2023Article
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- Mussel-inspired biomaterials: From chemistry to clinic.Bioengineering & translational medicine · 2022Review
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- Effects of Airborne Nanoparticles on the Nervous System: Amyloid Protein Aggregation, Neurodegeneration and Neurodegenerative Diseases.Nanomaterials (Basel, Switzerland) · 2020Review
- PAH SORPTION TO NANOPLASTICS AND THE TROJAN HORSE EFFECT AS DRIVERS OF MITOCHONDRIAL TOXICITY AND PAH LOCALIZATION IN ZEBRAFISH.Frontiers in environmental science · 2020Article
- Biological interaction levels of zinc oxide nanoparticles; lettuce seeds as case study.Heliyon · 2020Article
- Fluorescent PCDTBT Nanoparticles with Tunable Size for Versatile Bioimaging.Materials (Basel, Switzerland) · 2019Article
- Imaging modification of colon carcinoma cells exposed to lipid based nanovectors for drug delivery: a scanning electron microscopy investigation.RSC advances · 2019Article
- In Vitro Methods for Assessing Nanoparticle Toxicity.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Impact of nanoparticle surface functionalization on the protein corona and cellular adhesion, uptake and transport.Journal of nanobiotechnology · 2018Article
- An updated review of the genotoxicity of respirable crystalline silica.Particle and fibre toxicology · 2018Review
- Crucial role of chelatable iron in silver nanoparticles induced DNA damage and cytotoxicity.Redox biology · 2018Article
- Local raster image correlation spectroscopy generates high-resolution intracellular diffusion maps.Communications biology · 2018Article
- Anti-amyloid compounds protect from silica nanoparticle-induced neurotoxicity in the nematode C. elegans.Nanotoxicology · 2016Article
- An Intermittent Model for Intracellular Motions of Gold Nanostars by k-Space Scattering Image Correlation.Biophysical journal · 2015Article
- Functionalized Buckyballs for Visualizing Microbial Species in Different States and Environments.Scientific reports · 2015Article
- United polarizable multipole water model for molecular mechanics simulation.The Journal of chemical physics · 2015Article
- Tissue distribution and excretion kinetics of orally administered silica nanoparticles in rats.International journal of nanomedicine · 2014Article
- Nanotechnology in drug delivery: the need for more cell culture based studies in screening.Chemistry Central journal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding of nanoparticle-bio-interactions within living cells requires knowledge about the dynamic behavior of nanomaterials during their cellular uptake, intracellular traffic and mutual reactions with cell organelles. Here, we introduce a protocol of combined kinetic imaging techniques that enables investigation of exemplary fluorochrome-labelled nanoparticles concerning their intracellular fate. By time-lapse confocal microscopy we observe fast, dynamin-dependent uptake of polystyrene and silica nanoparticles via the cell membrane within seconds. Fluorescence recovery after photobleaching (FRAP) experiments reveal fast and complete exchange of the investigated nanoparticles at mitochondria, cytoplasmic vesicles or the nuclear envelope. Nuclear translocation is observed within minutes by free diffusion and active transport. Fluorescence correlation spectroscopy (FCS) and raster image correlation spectroscopy (RICS) indicate diffusion coefficients of polystyrene and silica nanoparticles in the nucleus and the cytoplasm that are consistent with particle motion in living cells based on diffusion. Determination of the apparent hydrodynamic radii by FCS and RICS shows that nanoparticles exert their cytoplasmic and nuclear effects mainly as mobile, monodisperse entities. Thus, a complete toolkit of fluorescence fluctuation microscopy is presented for the investigation of nanomaterial biophysics in subcellular microenvironments that contributes to develop a framework of intracellular nanoparticle delivery routes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.