ArticlePloS one2013
In vivo optical imaging of interscapular brown adipose tissue with (18)F-FDG via Cerenkov luminescence imaging.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
- Illuminating the metabolic sink: Optical probes for in vivo thermogenic fat imaging.Materials today. Bio · 2026Review
- Molecular Afterglow of Lophine-Based Luminophore and Its Imaging Applications.Angewandte Chemie (International ed. in English) · 2025Article
- In vivo three-dimensional brain imaging with chemiluminescence probes in Alzheimer's disease models.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A Photolabile Curcumin-Diazirine Analogue Enables Phototherapy with Physically and Molecularly Produced Light for Alzheimer's Disease Treatment.Angewandte Chemie (International ed. in English) · 2023Article
- Practical Guidance for Developing Small-Molecule Optical Probes for In Vivo Imaging.Molecular imaging and biology · 2023Review
- Imaging Brown Adipose Tissue with TSPO PET Tracers in Preclinical Animal Studies.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A novel monoacylglycerol lipase-targetedActa pharmacologica Sinica · 2022Article
- Cerenkov luminescence imaging of interscapular brown adipose tissue using a TSPO-targeting PET probe in the UCP1 ThermoMouse.Theranostics · 2022Article
- Molecular Imaging of Brown Adipose Tissue Mass.International journal of molecular sciences · 2021Review
- Glutamatergic Receptors Modulate Normoxic but Not Hypoxic Ventilation and Metabolism in Naked Mole Rats.Frontiers in physiology · 2019Article
- Cherenkov luminescence imaging is a fast and relevant preclinical tool to assess tumour hypoxia in vivo.EJNMMI research · 2018Article
- Dynamic imaging of the effect of mesenchymal stem cells on osteoclast precursor cell chemotaxis for bone defects in the mouse skull.Journal of dental sciences · 2018Article
- PET Imaging of Human Brown Adipose Tissue with the TSPO Tracer [Molecular imaging and biology · 2018Article
- Spectral Unmixing Imaging for Differentiating Brown Adipose Tissue Mass and Its Activation.Contrast media & molecular imaging · 2018Article
- Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Non-invasive Imaging Methods for Brown Adipose Tissue Detection and Function Evaluation.Internal medicine: open access · 2018Article
- Cerenkov luminescence imaging: physics principles and potential applications in biomedical sciences.EJNMMI physics · 2017Review
- Cerenkov Radiation-Induced Photoimmunotherapy withJournal of nuclear medicine : official publication, Society of Nuclear Medicine · 2017Article
- Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram andScientific reports · 2017Article
- Differentiating brown and white adipose tissues by high-resolution diffusion NMR spectroscopy.Journal of lipid research · 2017Article
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
objectiveBrown adipose tissue (BAT), a specialized tissue for thermogenesis, plays important roles for metabolism and energy expenditure. Recent studies validated BAT's presence in human adults, making it an important re-emerging target for various pathologies. During this validation, PET images with (18)F-FDG showed significant uptake of (18)F-FDG by BAT under certain conditions. Here, we demonstrated that Cerenkov luminescence imaging (CLI) using (18)F-FDG could be utilized for in vivo optical imaging of BAT in mice.
methodsMice were injected with (18)F-FDG and imaged 60 minutes later with open filter and 2 minute acquisition. In vivo activation of BAT was performed by norepinephrine and cold treatment under isoflurane or ketamine anesthesia. Spectral unmixing and 3D imaging reconstruction were conducted with multiple-filter CLI images.
results1) It was feasible to use CLI with (18)F-FDG to image interscapular BAT in mice, with the majority of the signal (>85%) at the interscapular site originating from BAT; 2) The method was reliable because excellent correlations between in vivo CLI, ex vivo CLI, and ex vivo radioactivity were observed; 3) CLI could be used for monitoring BAT activation under different conditions; 4) CLI signals from the group under short-term isoflurane anesthesia were significantly higher than that from the group under long-term anesthesia; 5) The CLI spectrum of (18)F-FDG with a peak at 640 nm in BAT after spectral unmixing reflected the actual context of BAT; 6) Finally 3D reconstruction images showed excellent correlation between the source of the light signal and the location and physical shape of BAT.
conclusionCLI with (18)F-FDG is a feasible and reliable method for imaging BAT in mice. Compared to PET imaging, CLI is significantly cheaper, faster for 2D planar imaging and easier to use. We believe that this method could be used as an important tool for researchers investigating BAT.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.