ArticlePloS one2013
Identification of genes and pathways related to phenol degradation in metagenomic libraries from petroleum refinery wastewater.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 76 citations in OpenAlex.
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- Genome mining the black-yeast Aureobasidium pullulans NRRL 62031 for biotechnological traits.BMC genomics · 2025Article
- Nexus of Soil Microbiomes, Genes, Classes of Carbon Substrates, and Biotransformation of Fluorotelomer-Based Precursors.Environmental science & technology · 2024Article
- Meta-omics assisted microbial gene and strain resources mining in contaminant environment.Engineering in life sciences · 2024Review
- XTT assay for detection of bacterial metabolic activity in water-based polyester polyurethane.PloS one · 2024Article
- Kinetic characterization of a new phenol degrading Acinetobacter towneri strain isolated from landfill leachate treating bioreactor.World journal of microbiology & biotechnology · 2023Article
- Genome-resolved analyses of oligotrophic groundwater microbial communities along phenol pollution in a continuous-flow biodegradation model system.Frontiers in microbiology · 2023Article
- Review
- Characterization and Expression Analysis of Extradiol and Intradiol Dioxygenase of Phenol-Degrading Haloalkaliphilic Bacterial Isolates.Current microbiology · 2022Article
- Microbe-assisted phytoremediation of environmental pollutants and energy recycling in sustainable agriculture.Archives of microbiology · 2021Review
- Whole genome strategies and bioremediation insight into dehalogenase-producing bacteria.Molecular biology reports · 2021Review
- Definition of the Metagenomic Profile of Ocean Water Samples From the Gulf of Mexico Based on Comparison With Reference Samples From Sites Worldwide.Frontiers in microbiology · 2021Article
- Highly Promiscuous Oxidases Discovered in the Bovine Rumen Microbiome.Frontiers in microbiology · 2018Article
- Metagenomic Approaches for Understanding New Concepts in Microbial Science.International journal of genomics · 2018Review
- Metatranscriptome Analysis Deciphers Multifunctional Genes and Enzymes Linked With the Degradation of Aromatic Compounds and Pesticides in the Wheat Rhizosphere.Frontiers in microbiology · 2018Article
- Degradation of phenol via ortho-pathway by Kocuria sp. strain TIBETAN4 isolated from the soils around Qinghai Lake in China.PloS one · 2018Article
- Biodegradation, Biosorption of Phenanthrene and Its Trans-Membrane Transport by Massilia sp. WF1 and Phanerochaete chrysosporium.Frontiers in microbiology · 2016Article
- Biostimulation of Indigenous Microbial Community for Bioremediation of Petroleum Refinery Sludge.Frontiers in microbiology · 2016Article
- Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two fosmid libraries, totaling 13,200 clones, were obtained from bioreactor sludge of petroleum refinery wastewater treatment system. The library screening based on PCR and biological activity assays revealed more than 400 positive clones for phenol degradation. From these, 100 clones were randomly selected for pyrosequencing in order to evaluate the genetic potential of the microorganisms present in wastewater treatment plant for biodegradation, focusing mainly on novel genes and pathways of phenol and aromatic compound degradation. The sequence analysis of selected clones yielded 129,635 reads at an estimated 17-fold coverage. The phylogenetic analysis showed Burkholderiales and Rhodocyclales as the most abundant orders among the selected fosmid clones. The MG-RAST analysis revealed a broad metabolic profile with important functions for wastewater treatment, including metabolism of aromatic compounds, nitrogen, sulphur and phosphorus. The predicted 2,276 proteins included phenol hydroxylases and cathecol 2,3- dioxygenases, involved in the catabolism of aromatic compounds, such as phenol, byphenol, benzoate and phenylpropanoid. The sequencing of one fosmid insert of 33 kb unraveled the gene that permitted the host, Escherichia coli EPI300, to grow in the presence of aromatic compounds. Additionally, the comparison of the whole fosmid sequence against bacterial genomes deposited in GenBank showed that about 90% of sequence showed no identity to known sequences of Proteobacteria deposited in the NCBI database. This study surveyed the functional potential of fosmid clones for aromatic compound degradation and contributed to our knowledge of the biodegradative capacity and pathways of microbial assemblages present in refinery wastewater treatment system.
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