ArticlePloS one2013
1,25-dihydroxyvitamin D3 protects against macrophage-induced activation of NFκB and MAPK signalling and chemokine release in human adipocytes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
69 citing papers in PubMed, 1 synthesis or guideline pooled it, 105 citations in OpenAlex.
- Pooled it
- Vitamin D and nonalcoholic fatty liver disease in children: a randomized controlled clinical trial.European journal of pediatrics · 2022Trial
- Vitamin D as a Regulator of the Biological Clock-Implications for Circadian-Metabolic Dysregulation.International journal of molecular sciences · 2026Review
- Cholecalciferol (vitamin D3) is an agonist of the Alzheimer's disease-associated immune receptor TREM2.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2026Article
- Phenotype-specific 25-hydroxyvitamin D and fat-soluble vitamin profiles in girls with pediatric endocrine disorders: a large cross-sectional study.Frontiers in nutrition · 2026Article
- Review
- Effects of Dietary Vitamin D Levels on Markers Related to Amyloidogenesis and Neuroinflammation in db/db Mice.Nutrients · 2025Article
- The potential role of 1,25(OH)Journal of inflammation (London, England) · 2025Review
- Antioxidant Supplementation in Childhood Obesity: A Path to Improved Metabolic Health?Antioxidants (Basel, Switzerland) · 2025Review
- Exploring the anti-inflammatory potential of vitamin D in cardiometabolic diseases.Metabolism open · 2025Article
- Vitamin D Enhancement of Adipose Biology: Implications on Obesity-Associated Cardiometabolic Diseases.Nutrients · 2025Review
- Vitamin D Attenuates Non-Alcoholic Fatty Liver Disease in High-Fat Diet-Induced Obesity Murine Model.Yonsei medical journal · 2025Article
- Vitamin D alleviates obesity-related metabolic abnormalities by modulating the gut microbiota in older female mice on a high-fat diet.Frontiers in cellular and infection microbiology · 2025Article
- The Anti-Inflammatory Roles of Vitamin D for Improving Human Health.Current issues in molecular biology · 2024Review
- Enhanced Metabolic Effects of Fish Oil When Combined with Vitamin D in Diet-Induced Obese Male Mice.Biomolecules · 2024Article
- 1,25-dihydroxyvitamin DNutrition research and practice · 2024Article
- Effects of anNutrition research and practice · 2024Article
- Transcriptomics identifies blunted immunomodulatory effects of vitamin D in people with multiple sclerosis.Scientific reports · 2024Article
- Confronting the global obesity epidemic: investigating the role and underlying mechanisms of vitamin D in metabolic syndrome management.Frontiers in nutrition · 2024Review
- From the Sun to the Cell: Examining Obesity through the Lens of Vitamin D and Inflammation.Metabolites · 2023Review
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Increased accumulation of macrophages in adipose tissue in obesity is linked to low-grade chronic inflammation, and associated with features of metabolic syndrome. Vitamin D3 may have immunoregulatory effects and reduce adipose tissue inflammation, although the molecular mechanisms remain to be established. This study investigated the effects of vitamin D3 on macrophage-elicited inflammatory responses in cultured human adipocytes, particularly the signalling pathways involved. Macrophage-conditioned (MC) medium (25% with adipocyte maintenance media) markedly inhibited protein expression of the nuclear factor-κB (NFκB) subunit inhibitor κBα (IκBα) (71%, P<0.001) and increased NFκB p65 (1.5-fold, P = 0.026) compared with controls. Treatment with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) abolished macrophage-induced activation of NFκB signalling by increasing IκBα expression (2.7-fold, P = 0.005) and reducing NFκB p65 phosphorylation (68%; P<0.001). The mitogen-activated protein kinase (MAPK) signalling was activated by MC medium, which was also blunted by 1,25(OH)2D3 with a downregulation of phosphorylated p38 MAPK (32%, P = 0.005) and phosphorylated Erk1/2 (49%, P = 0.001). Furthermore, MC medium (12.5% or 25%) dose-dependently upregulated secretion of key proinflammatory chemokines/cytokines (22-368-fold; all P<0.001) and this was significantly decreased by 1,25(OH)2D3: IL-8 (61% and 31%, P<0.001), MCP-1 (37%, P<0.001 and 36%, P = 0.002), RANTES (78% and 62%, P<0.001) and IL-6 (29%, P<0.001 and 34%, P = 0.019). Monocyte migration-elicited by adipocytes treated with 1,25(OH)2D3 was also reduced (up to 25%, P<0.001). In conclusion, vitamin D3 could be anti-inflammatory in adipose tissue, decreasing macrophage-induced release of chemokines and cytokines by adipocytes and the chemotaxis of monocytes. Our data suggests these effects are mediated by inhibition of the NFκB and MAPK signalling pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.