ArticlePloS one2013
A novel flow cytometric hemozoin detection assay for real-time sensitivity testing of Plasmodium falciparum.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Serum lipids and lipoproteins in malaria--a systematic review and meta-analysis.Malaria journal · 2013Pooled it
- Next Generation Chemiluminescent Probes for Antimalarial Drug Discovery.ACS infectious diseases · 2024Article
- Trio fluorophore-based phenotypic assay for the detection of artemisinin-induced growth-arrested Plasmodium falciparum in human erythrocytes.Scientific reports · 2024Article
- Morphometric and molecular characterization of an unpigmented haemosporidian parasite in the Neotropical turnip-tailed gecko (Parasitology · 2023Article
- Novel stem cell technologies are powerful tools to understand the impact of human factors onFrontiers in cellular and infection microbiology · 2023Article
- Dissecting The role ofEmerging microbes & infections · 2022Article
- Review of Microdevices for Hemozoin-Based Malaria Detection.Biosensors · 2022Review
- Field validation of a magneto-optical detection device (Gazelle) for portable point-of-care Plasmodium vivax diagnosis.PloS one · 2021Article
- Highly Sensitive and Rapid Characterization of the Development of Synchronized Blood Stage Malaria Parasites Via Magneto-Optical Hemozoin Quantification.Biomolecules · 2019Article
- Diamond Magnetic Microscopy of Malarial Hemozoin Nanocrystals.Physical review applied · 2019Article
- Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.Chemical reviews · 2019Review
- Development of Electrochemical Nanosensor for the Detection of Malaria Parasite in Clinical Samples.Frontiers in chemistry · 2019Article
- Development in Assay Methods forFrontiers in pharmacology · 2017Review
- Hemozoin detection may provide an inexpensive, sensitive, 1-minute malaria test that could revolutionize malaria screening.Expert review of anti-infective therapy · 2016Article
- Efficient monitoring of the blood-stage infection in a malaria rodent model by the rotating-crystal magneto-optical method.Scientific reports · 2016Article
- Flow Cytometry for Antimalarial Drug Testing: More than Meets the Eye.Journal of clinical microbiology · 2016Article
- Quantification of Plasmodium ex vivo drug susceptibility by flow cytometry.Malaria journal · 2015Article
- Assessing anti-malarial drug effects ex vivo using the haemozoin detection assay.Malaria journal · 2015Article
- Limitations of haemozoin-based diagnosis of Plasmodium falciparum using dark-field microscopy.Malaria journal · 2014Article
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resistance of Plasmodium falciparum to almost all antimalarial drugs, including the first-line treatment with artemisinins, has been described, representing an obvious threat to malaria control. In vitro antimalarial sensitivity testing is crucial to detect and monitor drug resistance. Current assays have been successfully used to detect drug effects on parasites. However, they have some limitations, such as the use of radioactive or expensive reagents or long incubation times. Here we describe a novel assay to detect antimalarial drug effects, based on flow cytometric detection of hemozoin (Hz), which is rapid and does not require any additional reagents. Hz is an optimal parasite maturation indicator since its amount increases as the parasite matures. Due to its physical property of birefringence, Hz depolarizes light, hence it can be detected using optical methods such as flow cytometry. A common flow cytometer was adapted to detect light depolarization caused by Hz. Synchronized in vitro cultures of P. falciparum were incubated for 48 hours with several antimalarial drugs. Analysis of depolarizing events, corresponding to parasitized red blood cells containing Hz, allowed the detection of parasite maturation. Moreover, chloroquine resistance and the inhibitory effect of all antimalarial drugs tested, except for pyrimethamine, could be determined as early as 18 to 24 hours of incubation. At 24 hours incubation, 50% inhibitory concentrations (IC50) were comparable to previously reported values. These results indicate that the reagent-free, real-time Hz detection assay could become a novel assay for the detection of drug effects on Plasmodium falciparum.
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