ArticlePloS one2013
Molecular and immunological characterization of ragweed (Ambrosia artemisiifolia L.) pollen after exposure of the plants to elevated ozone over a whole growing season.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 70 citations in OpenAlex.
- Common Ragweed Allergy Under Global Change Linking Invasion-Driven Aeroallergen Exposure with Molecular Sensitization and Allergic Airway Disease.Life (Basel, Switzerland) · 2026Review
- The role of pectate lyases in development and stress tolerance in plants.Molecular biology reports · 2025Review
- Environmental drivers of the allergenic load caused by Ambrosia artemisiifolia pollen and its major allergen Amb a 1 in the atmosphere.International journal of biometeorology · 2025Article
- Review
- Association between climate indicators and hay fever incidence in children and adolescents in Freiburg, Germany.Frontiers in public health · 2025Article
- Patterns of Phenolic Compounds inPlants (Basel, Switzerland) · 2023Article
- Short-Term Exposure ofPlants (Basel, Switzerland) · 2022Article
- Article
- Competing Bioaerosols May Influence the Seasonality of Influenza-Like Illnesses, including COVID-19. The Chicago Experience.Pathogens (Basel, Switzerland) · 2021Article
- Atmospheric pollutants and their association with olive and grass aeroallergen concentrations in Córdoba (Spain).Environmental science and pollution research international · 2020Article
- Initiating pollen sensitization - complex source, complex mechanisms.Clinical and translational allergy · 2020Review
- Impact of Climate Change on Pollen and Respiratory Disease.Current allergy and asthma reports · 2018Review
- POLLAR: Impact of air POLLution on Asthma and Rhinitis; a European Institute of Innovation and Technology Health (EIT Health) project.Clinical and translational allergy · 2018Review
- Modeling pollen-mediated gene flow from glyphosate-resistant to -susceptible giant ragweed (Ambrosia trifida L.) under field conditions.Scientific reports · 2017Article
- Plant pectin acetylesterase structure and function: new insights from bioinformatic analysis.BMC genomics · 2017Article
- Air Pollution and Climate Change Effects on Allergies in the Anthropocene: Abundance, Interaction, and Modification of Allergens and Adjuvants.Environmental science & technology · 2017Article
- T Cell Epitope-Containing Domains of Ragweed Amb a 1 and Mugwort Art v 6 Modulate Immunologic Responses in Humans and Mice.PloS one · 2017Article
- Analysis of morphological and molecular composition changes in allergenic Artemisia vulgaris L. pollen under traffic pollution using SEM and FTIR spectroscopy.Environmental science and pollution research international · 2016Article
- Is ragweed pollen allergenicity governed by environmental conditions during plant growth and flowering?Scientific reports · 2016Article
- Effects of NO2 and Ozone on Pollen Allergenicity.Frontiers in plant science · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Climate change and air pollution, including ozone is known to affect plants and might also influence the ragweed pollen, known to carry strong allergens. We compared the transcriptome of ragweed pollen produced under ambient and elevated ozone by 454-sequencing. An enzyme-linked immunosorbent assay (ELISA) was carried out for the major ragweed allergen Amb a 1. Pollen surface was examined by scanning electron microscopy and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and phenolics were analysed by high-performance liquid chromatography. Elevated ozone had no influence on the pollen size, shape, surface structure or amount of phenolics. ATR-FTIR indicated increased pectin-like material in the exine. Transcriptomic analyses showed changes in expressed-sequence tags (ESTs), including allergens. However, ELISA indicated no significantly increased amounts of Amb a 1 under elevated ozone concentrations. The data highlight a direct influence of ozone on the exine components and transcript level of allergens. As the total protein amount of Amb a 1 was not altered, a direct correlation to an increased risk to human health could not be derived. Additional, the 454-sequencing contributes to the identification of stress-related transcripts in mature pollen that could be grouped into distinct gene ontology terms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.