ArticlePloS one2013
PIN2 turnover in Arabidopsis root epidermal cells explored by the photoconvertible protein Dendra2.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Alterations in the proteomic profiles of Arabidopsis roots triggered by sodium chloride stress are exacerbated by impaired function of SYNAPTOTAGMIN 1.BMC plant biology · 2025Article
- Investigation ofPlant signaling & behavior · 2024Article
- Organelle Interactions in Plant Cells.Results and problems in cell differentiation · 2024Review
- SYNAPTOTAGMIN 4 is expressed mainly in the phloem and participates in abiotic stress tolerance in Arabidopsis.Frontiers in plant science · 2024Article
- A novel toolbox to record CLE peptide signaling.Frontiers in plant science · 2024Article
- Regulation of PIN-FORMED Protein Degradation.International journal of molecular sciences · 2023Review
- WAVY GROWTH Arabidopsis E3 ubiquitin ligases affect apical PIN sorting decisions.Nature communications · 2022Article
- Specific expression ofPlant signaling & behavior · 2021Article
- Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking.Plant physiology · 2021Article
- Auxin does not inhibit endocytosis of PIN1 and PIN2 auxin efflux carriers.Plant physiology · 2021Article
- Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport.The EMBO journal · 2021Article
- Abscisic Acid Regulates the Root Growth Trajectory by Reducing Auxin TransporterFrontiers in plant science · 2021Article
- Covalent Self-Labeling of Tagged Proteins with Chemical Fluorescent Dyes in BY-2 Cells and Arabidopsis Seedlings.The Plant cell · 2020Article
- Endosidin 2 accelerates PIN2 endocytosis and disturbs intracellular trafficking of PIN2, PIN3, and PIN4 but not of SYT1.PloS one · 2020Article
- Gateway-compatible vectors for functional analysis of proteins in cell type specific manner.Plant methods · 2020Article
- A Conditional Mutation inFrontiers in plant science · 2020Article
- Natural Auxin Does Not Inhibit Brefeldin A Induced PIN1 and PIN2 Internalization in Root Cells.Frontiers in plant science · 2019Article
- Mechanistic framework for cell-intrinsic re-establishment of PIN2 polarity after cell division.Nature plants · 2018Article
- Relative Contribution of PIN-Containing Secretory Vesicles and Plasma Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.International journal of molecular sciences · 2018Article
- A Cycloheximide-Sensitive Step in Transverse Microtubule Array Patterning.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The steady state level of integral membrane proteins is dependent on a strictly controlled delivery and removal. Here we show that Dendra2, a green-to-red photoconvertible fluorescent protein, is a suitable tool to study protein turnover in plants. We characterized the fluorescence properties of Dendra2 expressed either as a free protein or as a tag in Arabidopsis thaliana roots and optimized photoconversion settings to study protein turnover. Dendra2 was fused to the PIN2 protein, an auxin transporter in the root tip, and by time-lapse imaging and assessment of red and green signal intensities in the membrane after photoconversion we quantified directly and simultaneously the rate of PIN2 delivery of the newly synthesized protein into the plasma membrane as well as the disappearance of the protein from the plasma membrane due to degradation. Additionally we have verified several factors which are expected to affect PIN2 protein turnover and therefore potentially regulate root growth.
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