ArticlePLoS pathogens2013
Mutations in FLS2 Ser-938 dissect signaling activation in FLS2-mediated Arabidopsis immunity.
Article in PLoS pathogens, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 78 citations in OpenAlex.
- Puccinia triticina Effector Pt3863 Targets and Subverts TaRLCK176 to Suppress Wheat Resistance to Leaf Rust.Molecular plant pathology · 2026Article
- Flagellin sensing, signaling, and immune responses in plants.Plant communications · 2025Review
- Differential phosphorylation of receptor kinase SlLYK4 mediates immune responses to bacterial and fungal pathogens in tomato.Science advances · 2025Article
- A novel LRR receptor-like kinase BRAK reciprocally phosphorylates PSKR1 to enhance growth and defense in tomato.The EMBO journal · 2024Article
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- Allosteric activation of the co-receptor BAK1 by the EFR receptor kinase initiates immune signaling.eLife · 2024Article
- Mining of Minor Disease Resistance Genes inInternational journal of molecular sciences · 2023Article
- Unraveling the Molecular Mechanisms of Tomatoes' Defense againstPlants (Basel, Switzerland) · 2023Article
- Tomato LysM receptor kinase 4 mediates chitin-elicited fungal resistance in both leaves and fruit.Horticulture research · 2023Article
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- Thirty years of resistance: Zig-zag through the plant immune system.The Plant cell · 2022Review
- Activation loop phosphorylation of a non-RD receptor kinase initiates plant innate immune signaling.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Understanding and Exploiting Post-Translational Modifications for Plant Disease Resistance.Biomolecules · 2021Review
- Molecular Characterization of Differences between the Tomato Immune Receptors Flagellin Sensing 3 and Flagellin Sensing 2.Plant physiology · 2020Article
- Plant G-protein signaling cascade and host defense.3 Biotech · 2020Review
- Article
- Sucrose-induced Receptor Kinase 1 is Modulated by an Interacting Kinase with Short Extracellular Domain.Molecular & cellular proteomics : MCP · 2019Article
- Crosstalk of the Brassinosteroid Signalosome with Phytohormonal and Stress Signaling Components Maintains a Balance between the Processes of Growth and Stress Tolerance.International journal of molecular sciences · 2018Review
- Extracellular ATP elicits DORN1-mediated RBOHD phosphorylation to regulate stomatal aperture.Nature communications · 2017Article
- SlBIR3 Negatively Regulates PAMP Responses and Cell Death in Tomato.International journal of molecular sciences · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flagellin-sensing 2 (FLS2) is a leucine-rich repeat/transmembrane domain/protein kinase (LRR-RLK) that is the plant receptor for bacterial flagellin or the flagellin-derived flg22 peptide. Previous work has shown that after flg22 binding, FLS2 releases BIK1 kinase and homologs and associates with BAK1 kinase, and that FLS2 kinase activity is critical for FLS2 function. However, the detailed mechanisms for activation of FLS2 signaling remain unclear. The present study initially identified multiple FLS2 in vitro phosphorylation sites and found that Serine-938 is important for FLS2 function in vivo. FLS2-mediated immune responses are abolished in transgenic plants expressing FLS2(S938A), while the acidic phosphomimic mutants FLS2(S938D) and FLS2(S938E) conferred responses similar to wild-type FLS2. FLS2-BAK1 association and FLS2-BIK1 disassociation after flg22 exposure still occur with FLS2(S938A), demonstrating that flg22-induced BIK1 release and BAK1 binding are not sufficient for FLS2 activity, and that Ser-938 controls other aspects of FLS2 activity. Purified BIK1 still phosphorylated purified FLS2(S938A) and FLS2(S938D) mutant kinase domains in vitro. Phosphorylation of BIK1 and homologs after flg22 exposure was disrupted in transgenic Arabidopsis thaliana plants expressing FLS2(S938A) or FLS2(D997A) (a kinase catalytic site mutant), but was normally induced in FLS2(S938D) plants. BIK1 association with FLS2 required a kinase-active FLS2, but FLS2-BAK1 association did not. Hence FLS2-BIK1 dissociation and FLS2-BAK1 association are not sufficient for FLS2-mediated defense activation, but the proposed FLS2 phosphorylation site Ser-938 and FLS2 kinase activity are needed both for overall defense activation and for appropriate flg22-stimulated phosphorylation of BIK1 and homologs.
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