ArticleJournal of clinical microbiology2013
Development and evaluation of a next-generation digital PCR diagnostic assay for ocular Chlamydia trachomatis infections.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 113 citations in OpenAlex.
- Does azithromycin given to women in labour decrease ocular bacterial infection in neonates? A double-blind, randomized trial.BMC infectious diseases · 2017Trial
- Prevention of bacterial infections in the newborn by pre-delivery administration of azithromycin: Study protocol of a randomized efficacy trial.BMC pregnancy and childbirth · 2015Trial
- Comparison of Methods for Assessing Chlamydia trachomatis Transmission Intensity: A Systematic Review.The American journal of tropical medicine and hygiene · 2026Article
- Article
- Laboratory detection methods for Chlamydia trachomatis infection in children: a review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Review
- Evolutionary dynamics in the genome of ocularMicrobial genomics · 2025Article
- Murine modeling of menstruation identifies immune correlates of protection during Chlamydia muridarum challenge.PLoS pathogens · 2025Article
- Trachoma.Lancet (London, England) · 2025Review
- Highly viable gastrointestinal Chlamydia trachomatis in women abstaining from receptive anal intercourse.Scientific reports · 2025Article
- Validation of a New Duplex Real-Time Polymerase Chain Reaction forDiagnostics (Basel, Switzerland) · 2024Article
- Genomic insights into local-scale evolution of ocularMicrobial genomics · 2024Article
- Multiplex ddPCR: A Promising Diagnostic Assay for Early Detection and Drug Monitoring in Bovine Theileriosis.Pathogens (Basel, Switzerland) · 2023Article
- Trachoma.Nature reviews. Disease primers · 2022Review
- The conjunctival microbiome before and after azithromycin mass drug administration for trachoma control in a cohort of Tanzanian children.Frontiers in public health · 2022Article
- Duplex dPCR System for Rapid Identification of Gram-Negative Pathogens in the Blood of Patients with Bloodstream Infection: A Culture-Independent Approach.Journal of microbiology and biotechnology · 2021Article
- Comparison of genovars and Chlamydia trachomatis infection loads in ocular samples from children in two distinct cohorts in Sudan and Morocco.PLoS neglected tropical diseases · 2021Article
- Microfluidic compartmentalization to identify gene biomarkers of infection.Biomicrofluidics · 2020Review
- Viability PCR shows that non-ocular surfaces could contribute to transmission of Chlamydia trachomatis infection in trachoma.PLoS neglected tropical diseases · 2020Article
- Ocular Chlamydia trachomatis infection and infectious load among pre-school aged children within trachoma hyperendemic districts receiving the SAFE strategy, Amhara region, Ethiopia.PLoS neglected tropical diseases · 2020Article
- Ocular Chlamydia trachomatis infection, anti-Pgp3 antibodies and conjunctival scarring in Vanuatu and Tarawa, Kiribati before antibiotic treatment for trachoma.The Journal of infection · 2020Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
Abstract
Droplet digital PCR (ddPCR) is an emulsion PCR process that performs absolute quantitation of nucleic acids. We developed a ddPCR assay for Chlamydia trachomatis infections and found it to be accurate and precise. Using PCR mixtures containing plasmids engineered to include the PCR target sequences, we were able to quantify with a dynamic range between 0.07 and 3,160 targets/μl (r(2) = 0.9927) with >95% confidence. Using 1,509 clinical conjunctival swab samples from a population in which trachoma is endemic in Guinea Bissau, we evaluated the specificity and sensitivity of the quantitative ddPCR assay in diagnosing ocular C. trachomatis infections by comparing the performances of ddPCR and the Roche Amplicor CT/NG test. We defined ddPCR tests as positive when we had ≥95% confidence in a nonzero estimate of target load. The sensitivity of ddPCR against Amplicor was 73.3% (95% confidence interval [CI], 67.9 to 78.7%), and specificity was 99.1% (95% CI, 98.6 to 99.6%). Negative and positive predictive values were 94.6% (95% CI, 93.4 to 95.8%) and 94.5% (95% CI, 91.3 to 97.7%), respectively. Based on Amplicor CT/NG testing, the estimated population prevalence of C. trachomatis ocular infection was ∼17.5%. Receiver-operator curve analysis was used to select critical cutoff values for use in clinical settings in which a balance between higher sensitivity and specificity is required. We concluded that ddPCR is an effective diagnostic technology suitable for both research and clinical use in diagnosing ocular C. trachomatis infections.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.