ArticleJournal of clinical microbiology2013
Time profile of viral DNA in aqueous humor samples of patients treated for varicella-zoster virus acute retinal necrosis by use of quantitative real-time PCR.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Clinical and Virological Characteristics and Prognostic Factors in Viral Necrotizing Retinitis.Journal of personalized medicine · 2022Article
- Comparative Efficacy of Topical Ophthalmic Ganciclovir and Oral Famciclovir in Cats with Experimental Ocular Feline Herpesvirus-1 Epithelial Infection.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2022Article
- Acute retinal necrosis: A mini review.Frontiers in ophthalmology · 2022Review
- Advances in the microbiological diagnosis of herpetic retinitis.Frontiers in ophthalmology · 2022Review
- Acute Retinal Necrosis: Clinical Features, Diagnostic Pitfalls, Treatment, and Outcome of an Insidious Disease in Children. Case Report and Review of the Literature.Frontiers in pediatrics · 2022Article
- Clinically applicable artificial intelligence algorithm for the diagnosis, evaluation, and monitoring of acute retinal necrosis.Journal of Zhejiang University. Science. B · 2021Article
- Ocular Involvement of SARS-CoV-2 in a Polish Cohort of COVID-19-Positive Patients.International journal of environmental research and public health · 2021Article
- Viral Loads in Ocular Fluids of Acute Retinal Necrosis Eyes Infected by Varicella-Zoster Virus Treated with Intravenous Acyclovir Treatment.Journal of clinical medicine · 2020Article
- Alpha herpes virus type and viral load in intraocular fluids in patients with acute retinal necrosis.BMJ open ophthalmology · 2019Article
- Real-time polymerase chain reaction in acute retinal necrosis following encephalitis.Indian journal of ophthalmology · 2018Article
- Polymerase chain reaction to search for Herpes viruses in uveitic and healthy eyes: a South African perspective.African health sciences · 2015Article
- Advances in the management of acute retinal necrosis.International ophthalmology clinics · 2015Review
- Perplexing Etiology of Acute Retinal Necrosis in a Renal Transplant Recipient.Indian journal of nephrologyArticle
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to evaluate the kinetics of varicella-zoster virus (VZV) loads using quantitative PCR (qPCR) in patients treated for acute retinal necrosis (ARN). Six patients (52 ± 13 years old) with ARN syndrome were consecutively studied. Aqueous humor (AH) was sampled from both eyes of all patients for qPCR evaluation. The patients were treated with intravenous acyclovir and intravitreal injections of antiviral drugs. The mean follow-up time was 17.6 ± 16.4 months. Main outcome measures were the numbers of viral genome copies in the AH, assessed using real-time qPCR with hydrolysis probe technology with a threshold of detection of 200 copies/ml. Two main portions of the viral load curves were observed for each patient: a plateau phase (27.8 ± 24.9 days) and a decrease in the number of viral genome copies. The mean baseline viral load was 3.4 × 10(7) ± 4.45 × 10(7) copies/ml (6 × 10(6) to 1.2 × 10(8) copies/ml). The viral load decreased according to a logarithmic model, with a 50% reduction obtained in 3 ± 0.7 days. There was a significant viral load (>102 copies/ml) at 50 days after the onset of treatment, despite antiviral drugs. qPCR use demonstrated reproducible VZV DNA kinetics with a two-phase evolution: a plateau followed by a logarithmic decrease. These data suggest that high-dosage antiviral therapy administered for the conventional 10-day duration is insufficient for most patients. This series of patients responded with a similar decrease in viral load once treatment was initiated, and the data from these patients may be used to predict the responses of future patients.
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