ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2013
Potentiation of inhibitory synaptic transmission by extracellular ATP in rat suprachiasmatic nuclei.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Cell Type-Specific Expression of Purinergic P2X Receptors in the Hypothalamus.International journal of molecular sciences · 2025Review
- Cortical astrocyte N-methyl-D-aspartate receptors influence whisker barrel activity and sensory discrimination in mice.Nature communications · 2024Article
- The circadian regulation of extracellular ATP.Purinergic signalling · 2023Review
- P2X2 receptors in pyramidal neurons are critical for regulating vulnerability to chronic stress.Theranostics · 2022Article
- Analysis of Spatial and Temporal Distribution of Purinergic P2 Receptors in the Mouse Hippocampus.International journal of molecular sciences · 2021Article
- Purinergic P2 and glutamate NMDA receptor coupling contributes to osmotically driven excitability in hypothalamic magnocellular neurosecretory neurons.The Journal of physiology · 2021Article
- Impaired calcium signaling in astrocytes modulates autism spectrum disorder-like behaviors in mice.Nature communications · 2021Article
- Astrocyte Circadian Timekeeping in Brain Health and Neurodegeneration.Advances in experimental medicine and biology · 2021Article
- The Role of Purinergic Receptors in the Circadian System.International journal of molecular sciences · 2020Review
- PJournal of pain research · 2020Article
- P2X2 Receptor Expression and Function Is Upregulated in the Rat Supraoptic Nucleus Stimulated Through Refeeding After Fasting.Frontiers in cellular neuroscience · 2019Article
- Cholecystokinin Switches the Plasticity of GABA Synapses in the Dorsomedial Hypothalamus via Astrocytic ATP Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- The circadian clock: A tale of genetic-electrical interplay and synaptic integration.Current opinion in physiology · 2018Article
- The Intertwined Roles of Circadian Rhythms and Neuronal Metabolism Fueling Drug Reward and Addiction.Current opinion in physiology · 2018Article
- Circadian ATP Release in Organotypic Cultures of the Rat Suprachiasmatic Nucleus Is Dependent on P2X7 and P2Y Receptors.Frontiers in pharmacology · 2018Article
- Article
- Time-of-day-dependent expression of purinergic receptors in mouse suprachiasmatic nucleus.Cell and tissue research · 2017Article
- The role of P2X3 receptors in bilateral masseter muscle allodynia in rats.Croatian medical journal · 2016Article
- Extracellular ATP and other nucleotides-ubiquitous triggers of intercellular messenger release.Purinergic signalling · 2016Review
- Purinergic signaling pathways in endocrine system.Autonomic neuroscience : basic & clinical · 2015Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hypothalamic suprachiasmatic nuclei (SCN), the circadian master clock in mammals, releases ATP in a rhythm, but the role of extracellular ATP in the SCN is still unknown. In this study, we examined the expression and function of ATP-gated P2X receptors (P2XRs) in the SCN neurons of slices isolated from the brain of 16- to 20-day-old rats. Quantitative RT-PCR showed that the SCN contains mRNA for P2X 1-7 receptors and several G-protein-coupled P2Y receptors. Among the P2XR subunits, the P2X2 > P2X7 > P2X4 mRNAs were the most abundant. Whole-cell patch-clamp recordings from SCN neurons revealed that extracellular ATP application increased the frequency of spontaneous GABAergic IPSCs without changes in their amplitudes. The effect of ATP appears to be mediated by presynaptic P2X2Rs because ATPγS and 2MeS-ATP mimics, while the P2XR antagonist PPADS blocks, the observed enhancement of the frequency of GABA currents. There were significant differences between two SCN regions in that the effect of ATP was higher in the ventrolateral subdivision, which is densely innervated from outside the SCN. Little evidence was found for the presence of P2XR channels in somata of SCN neurons as P2X2R immunoreactivity colocalized with synapsin and ATP-induced current was observed in only 7% of cells. In fura-2 AM-loaded slices, BzATP as well as ADP stimulated intracellular Ca(2+) increase, indicating that the SCN cells express functional P2X7 and P2Y receptors. Our data suggest that ATP activates presynaptic P2X2Rs to regulate inhibitory synaptic transmission within the SCN and that this effect varies between regions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.