ArticleNanoscale2013
Ultrasensitive single-nucleotide polymorphism detection using target-recycled ligation, strand displacement and enzymatic amplification.
Article in Nanoscale, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 48 citations in OpenAlex.
- Dual-probe ligation without PCR for fluorescent sandwich assay of EGFR nucleotide variants in magnetic gene capture platform.Mikrochimica acta · 2023Article
- G-Quadruplexes as An Alternative Recognition Element in Disease-Related Target Sensing.Molecules (Basel, Switzerland) · 2019Review
- Exonuclease III-Regulated Target Cyclic Amplification-Based Single Nucleotide Polymorphism Detection Using Ultrathin Ternary Chalcogenide Nanosheets.Frontiers in chemistry · 2019Article
- Turn-off colorimetric sensor for sequence-specific recognition of single-stranded DNA based upon Y-shaped DNA structure.Scientific reports · 2018Article
- Ultrasensitive optical biosensor for detection of miRNA-155 using positively charged Au nanoparticles.Scientific reports · 2018Article
- Ultraefficient Cap-Exchange Protocol To Compact Biofunctional Quantum Dots for Sensitive Ratiometric Biosensing and Cell Imaging.ACS applied materials & interfaces · 2017Article
- Thermal therapy with magnetic nanoparticles for cell destruction.Biomedical optics express · 2016Article
- Sensitive detection of miRNA by using hybridization chain reaction coupled with positively charged gold nanoparticles.Scientific reports · 2016Article
- Accurate Detection of Rifampicin-Resistant Mycobacterium Tuberculosis Strains.Sensors (Basel, Switzerland) · 2016Article
- Magnetic micro/nanoparticle flocculation-based signal amplification for biosensing.International journal of nanomedicine · 2016Article
- Nano-enabled bioanalytical approaches to ultrasensitive detection of low abundance single nucleotide polymorphisms.The Analyst · 2015Review
- Robust and specific ratiometric biosensing using a copper-free clicked quantum dot-DNA aptamer sensor.Nanoscale · 2013Article
- Sensitive, simultaneous quantitation of two unlabeled DNA targets using a magnetic nanoparticle-enzyme sandwich assay.Analytical chemistry · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
We report herein the development of a highly sensitive and selective approach for label-free DNA detection by combining target-recycled ligation (TRL), magnetic nanoparticle assisted target capture/separation, and efficient enzymatic amplification. We show that our approach can detect as little as 30 amol (600 fM in 50 μL) of unlabelled single-stranded DNA targets and offer an exquisitely high discrimination ratio (up to >380 fold with background correction) between a perfect-match cancer mutant and its single-base mismatch (wild-type) DNA target. Furthermore, it can quantitate the rare cancer mutant (KRAS codon 12) in a large excess of coexisting wild-type DNAs down to 0.75%. This sensor appears to be well-suited for sensitive SNP detection and a wide range of DNA mutation based diagnostic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.