ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2013
Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.).
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- The haplotype-resolved and near-telomere-to-telomere genome assembly for the autotetraploid alfalfa.Plant communications · 2026Article
- Chromosome-specific painting provides insights into the karyotype evolutionary direction and trajectory in the genusHorticulture research · 2026Article
- Oligo-FISH barcode chromosome identification system provides novel insights into the natural chromosome aberrations propensity in the autotetraploid cultivated alfalfa.Horticulture research · 2025Article
- Insight into the Chromosome Structure of the Cultivated Tetraploid Alfalfa (Plants (Basel, Switzerland) · 2020Article
- Karyotypic evolution of the Medicago complex: sativa-caerulea-falcata inferred from comparative cytogenetic analysis.BMC evolutionary biology · 2017Article
- Isolation and application of P genome-specific DNA sequences of Agropyron Gaertn. in Triticeae.Planta · 2017Article
- Isolation and characterization of chromosomal markers inMolecular cytogenetics · 2017Article
- Differential repetitive DNA composition in the centromeric region of chromosomes of Amazonian lizard species in the family Teiidae.Comparative cytogenetics · 2016Article
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Authors and funding
6 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Eleven tandemly repetitive sequences were identified from a Cot-1 library by FISH and sequence analysis of alfalfa (Medicago sativa). Five repetitive sequences (MsCR-1, MsCR-2, MsCR-3, MsCR-4, and MsCR-5) were centromeric or pericentromeric, of which three were satellite DNAs and two were minisatellite DNAs. Monomers of 144, 148, and 168 bp were identified in MsCR-1, MsCR-2, and MsCR-3, respectively, while 15 and 39 bp monomers were identified in MsCR-4 and MsCR-5, respectively. Three repetitive sequences were characterized as subtelomeric; one repetitive sequence, MsTR-1, had a 184 bp monomer, and two repetitive sequences had fragments of 204 and 327 bp. Sequence analysis revealed homology (70-80 %) between MsTR-1 and a highly repeated sequence (C300) isolated from M. ssp. caerulea. Three identified repetitive sequences produced hybridization signals at multiple sites in a few of the chromosomes; one repetitive sequence was identified as the E180 satellite DNA previously isolated from M. sativa, while the other 163 and 227 bp fragments had distinct sequences. Physical mapping of the repetitive sequences with double-target FISH revealed different patterns. Thus, nine novel tandemly repetitive sequences that can be adopted as distinct chromosome markers in alfalfa were identified in this study. Furthermore, the chromosome distribution of each sequence was well described. Though significant chromosome variations were detected within and between cultivars, a molecular karyotype of alfalfa was suggested with the chromosome markers we identified. Therefore, these novel chromosome markers will still be a powerful tool for genome composition analysis, phylogenetic studies, and breeding applications.
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