ArticleThe protein journal2013
Solubilization and identification of hen eggshell membrane proteins during different times of chicken embryo development using the proteomic approach.
Article in The protein journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 69 citations in OpenAlex.
- Article
- Impact of eggshell membrane on metabolism and cell adhesion in oxidatively stressed canine chondrocytes.Frontiers in veterinary science · 2024Article
- Microbially Induced Calcium Carbonate Precipitation byApplied and environmental microbiology · 2023Review
- Eggshell Membrane as a Biomaterial for Bone Regeneration.Polymers · 2023Review
- Advances in eggshell membrane separation and solubilization technologies.Frontiers in veterinary science · 2023Review
- Hatched Eggshell Membrane Can Be a Novel Source of Antioxidant Hydrolysates to Protect against HAntioxidants (Basel, Switzerland) · 2022Article
- Avian eggshell biomineralization: an update on its structure, mineralogy and protein tool kit.BMC molecular and cell biology · 2021Review
- Avian eggshell formation reveals a new paradigm for vertebrate mineralization via vesicular amorphous calcium carbonate.The Journal of biological chemistry · 2020Article
- Wavy small-diameter vascular graft made of eggshell membrane and thermoplastic polyurethane.Materials science & engineering. C, Materials for biological applications · 2020Article
- State-of-the-Art of Eggshell Waste in Materials Science: Recent Advances in Catalysis, Pharmaceutical Applications, and Mechanochemistry.Frontiers in bioengineering and biotechnology · 2020Review
- Identification and Characterization of Phosphoproteins in Somatic Embryogenesis Acquisition during Oil Palm Tissue Culture.Plants (Basel, Switzerland) · 2019Article
- The glycoproteins EDIL3 and MFGE8 regulate vesicle-mediated eggshell calcification in a new model for avian biomineralization.The Journal of biological chemistry · 2019Article
- Evaluation of Changes in the Expression Pattern of EDIL3 in Different Grades of Endometrial Cancer.Current pharmaceutical biotechnology · 2019Article
- Protein profiles of hatchery egg shell membrane.Proteome science · 2016Article
- Water-soluble egg membrane enhances the immunoactivating properties of anClinical, cosmetic and investigational dermatology · 2016Article
- Article
- Identifying specific proteins involved in eggshell membrane formation using gene expression analysis and bioinformatics.BMC genomics · 2015Article
- Extraction and identification of membrane proteins from black widow spider eggs.Dong wu xue yan jiu = Zoological research · 2015Article
- Proteomic analysis of quail calcified eggshell matrix: a comparison to chicken and turkey eggshell proteomes.Proteome science · 2015Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fertilized chicken egg is a unit of life. During hatching, transport of nutrients, including calcium, have been reported from the egg components to the developing embryo. Calcium is mobilized from the eggshell with the involvement of Ca(2+)-binding proteins. In addition, other unknown proteins may also play some important roles during embryo developing process. Therefore identification and prediction of biological functions of eggshell membrane (ESM) proteins during chick embryo development was conducted by proteome analysis. Comparison of different lysis solutions indicated that the highest ability to extract ESM proteins could be obtained with 1 % sodium dodecyl sulfate in 5 mM Tris-HCl buffer pH 8.8 containing 0.1 % 2-mercaptoethanol. In this study fertilized Cornish chicken eggs were incubated at 37 °C in humidified incubators for up to 21 days. At selected times (days 1, 9, 15 and 21), samples were taken and the ESMs were carefully separated by hand, washed with distilled water, and air-dried at room temperature. The ESM proteins were then solubilized and analyzed by proteome analysis. Sodium dodecyl sulfate polyacrylamide gel electrophoresis combined with high performance liquid chromatography and mass spectrometry revealed 62 proteins in the ESM; only keratin is known ESM protein, 8 of which are egg white proteins and related while 53 others have not previously been reported. Some differences in the types of proteins and their molecular functions were noted in ESM at different incubation times. One protein which was present only at days 15 and 21 of egg incubation was identified as a calcium binding protein i.e. EGF like repeats and discoidin I like domain 3 (EDIL3 homologous protein).
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Registered trials
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