ArticleMolecular microbiology2013
Bacterial DNA polymerases participate in oligonucleotide recombination.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
- Mycobacterium smegmatis NucS-promoted DNA mismatch repair involves limited resection by a 5'-3' exonuclease and is independent of homologous recombination and NHEJ.Nucleic acids research · 2024Article
- Balanced cell division is secured by two different regulatory sites in OxyS RNA.RNA (New York, N.Y.) · 2024Article
- Recombineering in Non-Model Bacteria.Current protocols · 2022Article
- Past, Present, and Future of Genome Modification inMicroorganisms · 2022Review
- Hesperetin Inhibits Expression of Virulence Factors and Growth ofInternational journal of molecular sciences · 2021Article
- Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells.PLoS genetics · 2020Article
- CRISPR/Cas9 recombineering-mediated deep mutational scanning of essential genes in Escherichia coli.Molecular systems biology · 2020Article
- A new recombineering system for precise genome-editing in Shewanella oneidensis strain MR-1 using single-stranded oligonucleotides.Scientific reports · 2019Article
- Article
- Directed evolution of multiple genomic loci allows the prediction of antibiotic resistance.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- A 'Semi-Protected Oligonucleotide Recombination' Assay for DNA Mismatch Repair in vivo Suggests Different Modes of Repair for Lagging Strand Mismatches.Nucleic acids research · 2017Article
- Article
- λ Recombination and Recombineering.EcoSal Plus · 2016Review
- The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli.Scientific reports · 2015Article
- Chassis optimization as a cornerstone for the application of synthetic biology based strategies in microbial secondary metabolism.Frontiers in microbiology · 2015Review
- Lambda red mediated gap repair utilizes a novel replicative intermediate in Escherichia coli.PloS one · 2015Article
- MODEST: a web-based design tool for oligonucleotide-mediated genome engineering and recombineering.Nucleic acids research · 2014Article
- Temperate phages acquire DNA from defective prophages by relaxed homologous recombination: the role of Rad52-like recombinases.PLoS genetics · 2014Article
- Recombination promoted by DNA viruses: phage λ to herpes simplex virus.Annual review of microbiology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Synthetic single-strand oligonucleotides (oligos) with homology to genomic DNA have proved to be highly effective for constructing designed mutations in targeted genomes, a process referred to as recombineering. The cellular functions important for this type of homologous recombination have yet to be determined. Towards this end, we have identified Escherichia coli functions that process the recombining oligo and affect bacteriophage λ Red-mediated oligo recombination. To determine the nature of oligo processing during recombination, each oligo contained multiple nucleotide changes: a single base change allowing recombinant selection, and silent changes serving as genetic markers to determine the extent of oligo processing during the recombination. Such oligos were often not incorporated into the host chromosome intact; many were partially degraded in the process of recombination. The position and number of these silent nucleotide changes within the oligo strongly affect both oligo processing and recombination frequency. Exonucleases, especially those associated with DNA Polymerases I and III, affect inheritance of the silent nucleotide changes in the oligos. We demonstrate for the first time that the major DNA polymerases (Pol I and Pol III) and DNA ligase are directly involved with oligo recombination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.