ArticlePloS one2013
Bacterial community profiling of milk samples as a means to understand culture-negative bovine clinical mastitis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
86 citing papers in PubMed.
- Comparative effectiveness of individualised homeopathy and antibiotics in the treatment of bovine clinical mastitis: randomised controlled trial.The Veterinary record · 2018Trial
- Probiotics as Sustainable Alternatives to Mitigate Antibiotic Resistance in Bovine Mastitis Management.Probiotics and antimicrobial proteins · 2026Review
- An ode to the 16S rRNA gene: its history, importance, caveats and future in microbiome research.Microbial genomics · 2026Review
- Variety of Culturable Bacteria Associated with Subclinical Mastitis in Dairy Cows, Based on the Simpson's and Shannon-Wiener Diversity Indices.Antibiotics (Basel, Switzerland) · 2026Article
- Microbiome of Bovine Milk and Factors Influencing Its Composition.Animals : an open access journal from MDPI · 2026Review
- Dynamics of the milk microbial community during subacute ruminal acidosis with or without intramammary lipopolysaccharide challenge in dairy cows.Animal microbiome · 2026Article
- Whole genome sequencing reveals environmental pathogen misidentification and potential for cross-phylum antimicrobial resistance gene transfer in bovine mastitis: a pilot genomic study.BMC veterinary research · 2026Article
- Longitudinal study of mammary microbiota dynamics and mastitis prevention in Holstein cows by dry-off strategy.Frontiers in veterinary science · 2026Article
- Heat stress compromises nutritional quality and flavor of bovine raw milk: Evidence from multi-omics analyses.Food chemistry: X · 2025Article
- An investigation into variations in the raw milk microbiota of dairy cows in Ningxia, China: effects of season, farm, parity, and health status.BMC veterinary research · 2025Article
- Akkermansia muciniphila-Derived Outer Membrane Vesicles as a Novel Therapeutic Approach for Mastitis: Insights From In Vitro and Vivo Studies.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Unravelling the complexity of bovine milk microbiome: insights into mastitis through enterotyping using full-length 16S-metabarcoding.Animal microbiome · 2024Article
- The occurrence ofmSystems · 2024Article
- An in vitro evaluation of the effect of antimicrobial treatment on bovine mammary microbiota.Scientific reports · 2024Article
- Four novel Acinetobacter lwoffii strains isolated from the milk of cows in China with subclinical mastitis.BMC veterinary research · 2024Article
- Microbial Evolution in Artisanal Pecorino-like Cheeses Produced from Two Farms Managing Two Different Breeds of Sheep (Comisana and Lacaune).Foods (Basel, Switzerland) · 2024Article
- Bovine milk microbiota: Key players, origins, and potential contributions to early-life gut development.Journal of advanced research · 2024Review
- Integrated analysis of transcriptome and milk metagenome in subclinical mastitic and healthy cows.Animal bioscience · 2024Article
- Article
- Milk microbiome in the first month of lactation and at weaning from ewes supplemented with zinc pre- and postpartum.Journal of animal science · 2024Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Inflammation and infection of bovine mammary glands, commonly known as mastitis, imposes significant losses each year in the dairy industry worldwide. While several different bacterial species have been identified as causative agents of mastitis, many clinical mastitis cases remain culture negative, even after enrichment for bacterial growth. To understand the basis for this increasingly common phenomenon, the composition of bacterial communities from milk samples was analyzed using culture independent pyrosequencing of amplicons of 16S ribosomal RNA genes (16S rDNA). Comparisons were made of the microbial community composition of culture negative milk samples from mastitic quarters with that of non-mastitic quarters from the same animals. Genomic DNA from culture-negative clinical and healthy quarter sample pairs was isolated, and amplicon libraries were prepared using indexed primers specific to the V1-V2 region of bacterial 16S rRNA genes and sequenced using the Roche 454 GS FLX with titanium chemistry. Evaluation of the taxonomic composition of these samples revealed significant differences in the microbiota in milk from mastitic and healthy quarters. Statistical analysis identified seven bacterial genera that may be mainly responsible for the observed microbial community differences between mastitic and healthy quarters. Collectively, these results provide evidence that cases of culture negative mastitis can be associated with bacterial species that may be present below culture detection thresholds used here. The application of culture-independent bacterial community profiling represents a powerful approach to understand long-standing questions in animal health and disease.
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