ArticleActa crystallographica. Section D, Biological crystallography2013
Towards protein-crystal centering using second-harmonic generation (SHG) microscopy.
Article in Acta crystallographica. Section D, Biological crystallography, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- On the use of dioxane as reference for determination of the hydrodynamic radius by NMR spectroscopy.Biophysical journal · 2024Article
- Deep learning applications in protein crystallography.Acta crystallographica. Section A, Foundations and advances · 2024Review
- Low-doseIUCrJ · 2020Article
- Visualization of protein crystals by high-energy phase-contrast X-ray imaging.Acta crystallographica. Section D, Structural biology · 2019Article
- DeepCentering: fully automated crystal centering using deep learning for macromolecular crystallography.Journal of synchrotron radiation · 2019Article
- Strategies for sample delivery for femtosecond crystallography.Acta crystallographica. Section D, Structural biology · 2019Review
- Morphology and structure of ZIF-8 during crystallisation measured by dynamic angle-resolved second harmonic scattering.Nature communications · 2018Article
- The complex analysis of X-ray mesh scans for macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2018Article
- Second harmonic generation correlation spectroscopy for characterizing translationally diffusing protein nanocrystals.Acta crystallographica. Section D, Structural biology · 2016Article
- A visible-light-excited fluorescence method for imaging protein crystals without added dyes.Journal of applied crystallography · 2016Article
- Intercalating dyes for enhanced contrast in second-harmonic generation imaging of protein crystals.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Identifying, studying and making good use of macromolecular crystals.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Kinetic trapping of metastable amino acid polymorphs.Journal of the American Chemical Society · 2014Article
- Approaches to automated protein crystal harvesting.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline.Journal of synchrotron radiation · 2013Article
- Exploiting Microbeams for Membrane Protein Structure Determination.Advances in experimental medicine and biologyArticle
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10 authors.
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Abstract
The potential of second-harmonic generation (SHG) microscopy for automated crystal centering to guide synchrotron X-ray diffraction of protein crystals was explored. These studies included (i) comparison of microcrystal positions in cryoloops as determined by SHG imaging and by X-ray diffraction rastering and (ii) X-ray structure determinations of selected proteins to investigate the potential for laser-induced damage from SHG imaging. In studies using β2 adrenergic receptor membrane-protein crystals prepared in lipidic mesophase, the crystal locations identified by SHG images obtained in transmission mode were found to correlate well with the crystal locations identified by raster scanning using an X-ray minibeam. SHG imaging was found to provide about 2 µm spatial resolution and shorter image-acquisition times. The general insensitivity of SHG images to optical scatter enabled the reliable identification of microcrystals within opaque cryocooled lipidic mesophases that were not identified by conventional bright-field imaging. The potential impact of extended exposure of protein crystals to five times a typical imaging dose from an ultrafast laser source was also assessed. Measurements of myoglobin and thaumatin crystals resulted in no statistically significant differences between structures obtained from diffraction data acquired from exposed and unexposed regions of single crystals. Practical constraints for integrating SHG imaging into an active beamline for routine automated crystal centering are discussed.
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