ArticleProceedings of the National Academy of Sciences of the United States of America2013
Juvenile hormone and its receptor, methoprene-tolerant, control the dynamics of mosquito gene expression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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87 citing papers in PubMed, 138 citations in OpenAlex.
- Immune-reproductive cross talk in mosquitoes: molecular pathways and energy homeostasis.Parasites & vectors · 2026Review
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- Nutritional and hormonal regulation of mitochondrial biogenesis drives fat body remodeling for reproductive competence.Journal of advanced research · 2026Article
- Characterization of the Crustacean Methyl Farnesoate Transcriptional Signaling Genes.International journal of molecular sciences · 2026Article
- Blood feeding, microbiota, and layered mosquito immunity: from pathway phenotypes to vector competence.Frontiers in immunology · 2026Review
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- 20E-inducedProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Effects of Trehalase on the Gene Expression of the Reproductive Regulation Pathway Network and Triglyceride Metabolism inInsects · 2025Article
- Lysyl-tRNA synthetase orchestrates translation and hormone signaling during reproduction in Aedes aegypti mosquitoes.Communications biology · 2025Article
- Juvenile hormone and BMP signaling modulate fat body cell fate during the transition of previtellogenic development to vitellogenesis.BMC biology · 2025Article
- Interactions of juvenile hormone, 20-hydroxyecdysone, developmental genes, and miRNAs during pupal development in Apis mellifera.Scientific reports · 2025Article
- UnveilingFrontiers in cellular and infection microbiology · 2025Article
- Methoprene-Tolerant (Met) Acts as Methyl Farnesoate Receptor to Regulate Larva Metamorphosis in Mud Crab,International journal of molecular sciences · 2024Article
- The accumulation of modular serine protease mediated by a novel circRNA sponging miRNA increases Aedes aegypti immunity to fungus.BMC biology · 2024Article
- Juvenile hormone receptor Methoprene tolerant: Functions and applications.Vitamins and hormones · 2023Article
- Involvement of Methoprene-tolerant and Krüppel homolog 1 in juvenile hormone-mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae).Archives of insect biochemistry and physiology · 2023Article
- Ecdysone signaling mediates the trade-off between immunity and reproduction via suppression of amyloids in the mosquito Aedes aegypti.PLoS pathogens · 2022Article
- Purification of an insect juvenile hormone receptor complex enables insights into its post-translational phosphorylation.The Journal of biological chemistry · 2021Article
- Hormonal regulation of microRNA expression dynamics in the gut of the yellow fever mosquitoRNA biology · 2021Article
- Juvenile hormone signaling promotes ovulation and maintains egg shape by inducing expression of extracellular matrix genes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
27 more citing papers are in PubMed but not listed here.
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8 authors at 4 institutions in 3 countries.
Funding
Abstract
Juvenile hormone III (JH) plays a key role in regulating the reproduction of female mosquitoes. Microarray time-course analysis revealed dynamic changes in gene expression during posteclosion (PE) development in the fat body of female Aedes aegypti. Hierarchical clustering identified three major gene clusters: 1,843 early-PE (EPE) genes maximally expressed at 6 h PE, 457 mid-PE (MPE) genes at 24 h PE, and 1,815 late-PE (LPE) genes at 66 h PE. The RNAi microarray screen for the JH receptor Methoprene-tolerant (Met) showed that 27% of EPE and 40% of MPE genes were up-regulated whereas 36% of LPE genes were down-regulated in the absence of this receptor. Met repression of EPE and MPE and activation of LPE genes were validated by an in vitro fat-body culture experiment using Met RNAi. Sequence motif analysis revealed the consensus for a 9-mer Met-binding motif, CACG(C)/TG(A)/G(T)/AG. Met-binding motif variants were overrepresented within the first 300 bases of the promoters of Met RNAi-down-regulated (LPE) genes but not in Met RNAi-up-regulated (EPE) genes. EMSAs using a combination of mutational and anti-Met antibody supershift analyses confirmed the binding properties of the Met consensus motif variants. There was a striking temporal separation of expression profiles among major functional gene groups, with carbohydrate, lipid, and xenobiotics metabolism belonging to the EPE and MPE clusters and transcription and translation to the LPE cluster. This study represents a significant advancement in the understanding of the regulation of gene expression by JH and its receptor Met during female mosquito reproduction.
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