ArticleThe Journal of infectious diseases2013
Highly sensitive detection of malaria parasitemia in a malaria-endemic setting: performance of a new loop-mediated isothermal amplification kit in a remote clinic in Uganda.
Article in The Journal of infectious diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 162 papers, 4 of them syntheses that pooled it.
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Who cites it
162 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Loop-mediated isothermal amplification (LAMP) test in the detection of uncomplicated malaria in pregnancy: a meta-analysis of diagnostic accuracy.Malaria journal · 2022Pooled it
- Global trend of Plasmodium malariae and Plasmodium ovale spp. malaria infections in the last two decades (2000-2020): a systematic review and meta-analysis.Parasites & vectors · 2021Pooled it
- Prevalence and proportion of Plasmodium spp. triple mixed infections compared with double mixed infections: a systematic review and meta-analysis.Malaria journal · 2020Pooled it
- Loop-mediated isothermal amplification (LAMP) test for diagnosis of uncomplicated malaria in endemic areas: a meta-analysis of diagnostic test accuracy.Malaria journal · 2020Pooled it
- Infant sex modifies associations between placental malaria and risk of malaria in infancy.Malaria journal · 2020Trial
- Trial
- The impact of gravidity, symptomatology and timing of infection on placental malaria.Malaria journal · 2020Trial
- Trial
- Impact of Microscopic and Submicroscopic Parasitemia During Pregnancy on Placental Malaria in a High-Transmission Setting in Uganda.The Journal of infectious diseases · 2019Trial
- Modeling Prevention of Malaria and Selection of Drug Resistance with Different Dosing Schedules of Dihydroartemisinin-Piperaquine Preventive Therapy during Pregnancy in Uganda.Antimicrobial agents and chemotherapy · 2019Trial
- Intermittent Preventive Treatment for Malaria in Pregnancy: Optimization of Target Concentrations of Dihydroartemisinin-Piperaquine.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Trial
- Intermittent Preventive Treatment With Dihydroartemisinin-Piperaquine for the Prevention of Malaria Among HIV-Infected Pregnant Women.The Journal of infectious diseases · 2017Trial
- Dihydroartemisinin-Piperaquine for the Prevention of Malaria in Pregnancy.The New England journal of medicine · 2016Trial
- Malaria serology for surveillance, insights into immunity, and vaccine development.Infection and immunity · 2026Review
- Colorimetric RT-LAMP for SARS-CoV-2 detection from nasopharyngeal swabs or crude saliva: a multicountry diagnostic accuracy study in Africa.The Lancet. Global health · 2025Article
- Recombinase-Aided Loop-Mediated Isothermal Amplification on Human Plasmodium knowlesi.The American journal of tropical medicine and hygiene · 2024Article
- Evaluation of a Point-of-Need Molecular Diagnostic Tool Coupled with Rapid DNA Extraction Methods for Visceral Leishmaniasis.Diagnostics (Basel, Switzerland) · 2023Article
- Rapid Detection of Malaria Based on Hairpin-Mediated Amplification and Lateral Flow Detection.Micromachines · 2023Article
- First field and laboratory evaluation of LAMP assay for malaria diagnosis in Cubal, Angola.Parasites & vectors · 2023Article
- A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites.The Analyst · 2023Article
102 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCurrent malaria diagnostic tests, including microscopy and antigen-detecting rapid tests, cannot reliably detect low-density infections. Molecular methods such as polymerase chain reaction (PCR) are highly sensitive but remain too complex for field deployment. A new commercial molecular assay based on loop-mediated isothermal amplification (LAMP) was assessed for field use.
methodsMalaria LAMP (Eiken Chemical, Japan) was evaluated for samples from 272 outpatients at a rural Ugandan clinic and compared with expert microscopy, nested PCR, and quantitative PCR (qPCR). Two technicians performed the assay after 3 days of training, using 2 alternative blood sample-preparation methods and visual interpretation of results by fluorescence assay.
resultsCompared with 3-well nested PCR, the sensitivity of both LAMP and single-well nested PCR was 90%; the microscopy sensitivity was 51%. For samples with a Plasmodium falciparum qPCR titer of ≥ 2 parasites/µL, LAMP sensitivity was 97.8% (95% confidence interval, 93.7%-99.5%). Most false-negative LAMP results involved samples with parasitemia levels detectable by 3-well nested PCR but very low or undetectable by qPCR.
conclusionsMalaria LAMP in a remote Ugandan clinic achieved sensitivity similar to that of single-well nested PCR in a United Kingdom reference laboratory. LAMP dramatically lowers the detection threshold achievable in malaria-endemic settings, providing a new tool for diagnosis, surveillance, and screening in elimination strategies.
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