ArticlePlant physiology2013
Sporopollenin biosynthetic enzymes interact and constitute a metabolon localized to the endoplasmic reticulum of tapetum cells.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Protein-Protein Interactions Modulate a Key Branch Point in Monoterpene Indole Alkaloid Biosynthesis.ACS chemical biology · 2026Article
- Developmentally regulated genes drive phylogenomic splits in ovule evolution.Nature communications · 2025Article
- Insights into the Molecular Basis of Pollen Coat Development and Its Role in Male Sterility.International journal of molecular sciences · 2025Review
- Transcriptome Remodeling inPlants (Basel, Switzerland) · 2024Article
- Immunoprecipitation and FRET-FLIM to Determine Metabolons on the Plant ER.Methods in molecular biology (Clifton, N.J.) · 2024Article
- CRISPR/Cas9-based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB-ZmTKPR1-1/-2 required for pollen exine formation in maize.Plant biotechnology journal · 2024Article
- Transcriptomics and metabolomics analyses reveal pollen abortion mechanism in alfalfa early stage male sterile lines.Frontiers in plant science · 2024Article
- Review on tapetal ultrastructure in angiosperms.Planta · 2023Review
- A novel 7-base pair deletion at a splice site in MS-2 impairs male fertility via premature tapetum degradation in common bean (Phaseolis vulgaris L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- OsCCRL1 is Essential for Phenylpropanoid Metabolism in Rice Anthers.Rice (New York, N.Y.) · 2023Article
- Transcriptomic and Proteomic Analyses of Celery Cytoplasmic Male Sterile Line and Its Maintainer Line.International journal of molecular sciences · 2023Article
- BrACOS5 mutations induced male sterility via impeding pollen exine formation in Chinese cabbage (Brassica rapa L. ssp. pekinensis).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Cold Stress Response Mechanisms in Anther Development.International journal of molecular sciences · 2022Review
- SWOLLEN TAPETUM AND STERILITY 1 is required for tapetum degeneration and pollen wall formation in rice.Plant physiology · 2022Article
- Fleshy or dry: transcriptome analyses reveal the genetic mechanisms underlying bract development in Ephedra.EvoDevo · 2022Article
- Overexpression of sesame polyketide synthase A leads to abnormal pollen development in Arabidopsis.BMC plant biology · 2022Article
- Global transcriptome analysis reveals potential genes associated with genic male sterility of rapeseed (Frontiers in plant science · 2022Article
- Iron insufficiency in floral buds impairs pollen development by disrupting tapetum function.The Plant journal : for cell and molecular biology · 2021Article
- Tetraketide α-pyrone reductases in sporopollenin synthesis pathway in Gerbera hybrida: diversification of the minor function.Horticulture research · 2021Article
- Loss of THIN EXINE2 disrupts multiple processes in the mechanism of pollen exine formation.Plant physiology · 2021Article
2 more citing papers are in PubMed but not listed here.
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The sporopollenin polymer is the major constituent of exine, the outer pollen wall. Recently fatty acid derivatives have been shown to be the precursors of sporopollenin building units. ACYL-COA SYNTHETASE, POLYKETIDE SYNTHASE A (PKSA) and PKSB, TETRAKETIDE α-PYRONE REDUCTASE1 (TKPR1) and TKPR2 have been demonstrated to be involved in sporopollenin biosynthesis in Arabidopsis (Arabidopsis thaliana). Here all these sporopollenin biosynthetic enzymes but TKPR2 have been immunolocalized to endoplasmic reticulum of anther tapetal cells. Pull-down experiments demonstrated that tagged recombinant proteins interacted to form complexes whose constituents were characterized by immunoblotting. In vivo protein interactions were evidenced by yeast (Saccharomyces cerevisiae) two-hybrid analysis and by fluorescence lifetime imaging microscopy/Förster resonance energy transfer studies in transgenic Nicotiana benthamiana, which were used to test the possibility that the enzymes interact to form a biosynthetic metabolon. Various pairs of proteins fused to two distinct fluorochromes were coexpressed in N. benthamiana leaf tissues and fluorescence lifetime imaging microscopy/Förster resonance energy transfer measurements demonstrated that proteins interacted pairwise in planta. Taken together, these results suggest the existence of a sporopollenin metabolon.
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