ArticleThe Journal of biological chemistry2013
Matrix metalloproteinase 8 (collagenase 2) induces the expression of interleukins 6 and 8 in breast cancer cells.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.
- The Role of MMP8 in Cancer: A Systematic Review.International journal of molecular sciences · 2019Pooled it
- Mast Cells Accumulate in the Stroma of Breast Adenocarcinoma and Secrete Pro-Inflammatory Cytokines and Tumor-Damaging Mediators: Could IL-37 and IL-38 Play an Anti-Tumor Role?International journal of molecular sciences · 2026Review
- Asparagine endopeptidase prompts breast cancer-related pericardial calcification by regulating IGF2 and integrin αvβ5.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- New Dimethylpyridine-3-Carboxamide Derivatives as MMP-13 Inhibitors with Anticancer Activity.Molecules (Basel, Switzerland) · 2025Article
- Salivary Biomarker Analysis to Distinguish Between Health and Periodontitis Status: A Preliminary Study.Dentistry journal · 2025Article
- Impact of antibiotic choice on immune response and antibiotic resistance development in piglets experimentally infected withFrontiers in cellular and infection microbiology · 2025Article
- The adhesion-GPCR ADGRF5 fuels breast cancer progression by suppressing the MMP8-mediated antitumorigenic effects.Cell death & disease · 2024Article
- Neutrophil-activating secretome characterizes palbociclib-induced senescence of breast cancer cells.Cancer immunology, immunotherapy : CII · 2024Article
- High-Affinity Host-Guest Recognition for Efficient Assembly and Enzymatic Responsiveness of DNA Nanostructures.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Article
- Clinical risk factors and blood protein biomarkers of 10-year pneumonia risk.medRxiv : the preprint server for health sciences · 2023Article
- Chromatin insulation orchestrates matrix metalloproteinase gene cluster expression reprogramming in aggressive breast cancer tumors.Molecular cancer · 2023Article
- Neutrophil metalloproteinase driven spleen damage hampers infection control of trypanosomiasis.Nature communications · 2023Article
- Immunomodulatory effects of trans-anethole-treated Staphylococcus aureus Newman strain.Scientific reports · 2023Article
- MMP-9/BDNF ratio predicts more severe COVID-19 outcomes.International journal of medical sciences · 2022Article
- The Molecular Pathways of Lung Damage by E-Cigarettes in Male Wistar Rats.Sultan Qaboos University medical journal · 2021Article
- A Mini Review: The Potential Biomarkers for Non-invasive Diagnosis of Pulpal Inflammation.Frontiers in dental medicine · 2021Article
- Practical context of enzymatic treatment for wound healing: A secreted protease approach (Review).Biomedical reports · 2020Review
- Improved mouse models and advanced genetic and genomic technologies for the study of neutrophils.Drug discovery today · 2020Review
- RNA cargos in extracellular vesicles derived from blood serum in pancreas associated conditions.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Matrix metalloproteinase 8 (MMP-8) is a tumor-suppressive protease that cleaves numerous substrates, including matrix proteins and chemokines. In particular, MMP-8 proteolytically activates IL-8 and, thereby, regulates neutrophil chemotaxis in vivo. We explored the effects of expression of either a WT or catalytically inactive (E198A) mutant version of MMP-8 in human breast cancer cell lines. Analysis of serum-free conditioned media from three breast cancer cell lines (MCF-7, SK-BR-3, and MDA-MB-231) expressing WT MMP-8 revealed elevated levels of IL-6 and IL-8. This increase was mirrored at the mRNA level and was dependent on MMP-8 catalytic activity. However, sustained expression of WT MMP-8 by breast cancer cells was non-permissive for long-term growth, as shown by reduced colony formation compared with cells expressing either control vector or E198A mutant MMP-8. In long-term culture of transfected MDA-MB-231 cells, expression of WT but not E198A mutant MMP-8 was lost, with IL-6 and IL-8 levels returning to base line. Rare clonal isolates of MDA-MB-231 cells expressing WT MMP-8 were generated, and these showed constitutively high levels of IL-6 and IL-8, although production of the interleukins was no longer dependent upon MMP-8 activity. These studies support a causal connection between MMP-8 activity and the IL-6/IL-8 network, with an acute response to MMP-8 involving induction of the proinflammatory mediators, which may in part serve to compensate for the deleterious effects of MMP-8 on breast cancer cell growth. This axis may be relevant to the recognized ability of MMP-8 to orchestrate the innate immune system in inflammation in vivo.
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