ArticleThe Journal of biological chemistry2013
Large terminase conformational change induced by connector binding in bacteriophage T7.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Dissecting the dsDNA viral packaging machinery: structural evolution and potential for therapeutics.Journal of virology · 2026Review
- Genome anchoring, retention, and release by neck proteins of Staphylococcus phage 812.Communications biology · 2026Article
- Variable Assembly and Procapsid Binding of Bacteriophage P22 Terminase Subunits in Solution.Pathogens (Basel, Switzerland) · 2024Article
- Observation of Bacteriophage Ultrastructure by Cryo-Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Viral Genomic DNA Packaging Machinery.Sub-cellular biochemistry · 2024Review
- Insights into a viral motor: the structure of the HK97 packaging termination assembly.Nucleic acids research · 2023Article
- Isolation, Characterization and Whole Genome Analysis of an Avian PathogenicVeterinary sciences · 2022Article
- Viral Small Terminase: A Divergent Structural Framework for a Conserved Biological Function.Viruses · 2022Review
- Characterization of a Novel Bacteriophage swi2 Harboring Two Lysins Can Naturally LyseFrontiers in microbiology · 2021Article
- Biophysical analysis of Pseudomonas-phage PaP3 small terminase suggests a mechanism for sequence-specific DNA-binding by lateral interdigitation.Nucleic acids research · 2020Article
- Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism.Nature communications · 2019Article
- Characterization and genome analysis of a novel bacteriophage vB_SpuP_Spp16 that infects Salmonella enterica serovar pullorum.Virus genes · 2019Article
- Review
- Structure of the large terminase from a hyperthermophilic virus reveals a unique mechanism for oligomerization and ATP hydrolysis.Nucleic acids research · 2017Article
- Physical and Functional Characterization of a Viral Genome Maturation Complex.Biophysical journal · 2017Article
- Portal protein functions akin to a DNA-sensor that couples genome-packaging to icosahedral capsid maturation.Nature communications · 2017Article
- Mechanisms of DNA Packaging by Large Double-Stranded DNA Viruses.Annual review of virology · 2015Review
- Thermodynamic Interrogation of the Assembly of a Viral Genome Packaging Motor Complex.Biophysical journal · 2015Article
- Architecture of the Complex Formed by Large and Small Terminase Subunits from Bacteriophage P22.Journal of molecular biology · 2015Article
- Nucleotide-type chemical shift assignment of the encapsulated 40 kbp dsDNA in intact bacteriophage T7 by MAS solid-state NMR.Journal of biomolecular NMR · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During bacteriophage morphogenesis DNA is translocated into a preformed prohead by the complex formed by the portal protein, or connector, plus the terminase, which are located at an especial prohead vertex. The terminase is a powerful motor that converts ATP hydrolysis into mechanical movement of the DNA. Here, we have determined the structure of the T7 large terminase by electron microscopy. The five terminase subunits assemble in a toroid that encloses a channel wide enough to accommodate dsDNA. The structure of the complete connector-terminase complex is also reported, revealing the coupling between the terminase and the connector forming a continuous channel. The structure of the terminase assembled into the complex showed a different conformation when compared with the isolated terminase pentamer. To understand in molecular terms the terminase morphological change, we generated the terminase atomic model based on the crystallographic structure of its phage T4 counterpart. The docking of the threaded model in both terminase conformations showed that the transition between the two states can be achieved by rigid body subunit rotation in the pentameric assembly. The existence of two terminase conformations and its possible relation to the sequential DNA translocation may shed light into the molecular bases of the packaging mechanism of bacteriophage T7.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.