ArticleBMC genetics2013
Stability of genomic imprinting in human induced pluripotent stem cells.
Article in BMC genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Culture-dependent decoupling ofEpigenetics · 2026Article
- Sex in cardiovascular disease: Why this biological variable should be considered in in vitro models.Science advances · 2024Review
- Epigenetic and Transcriptional Shifts in Human Neural Stem Cells after Reprogramming into Induced Pluripotent Stem Cells and Subsequent Redifferentiation.International journal of molecular sciences · 2024Article
- BrewerIX enables allelic expression analysis of imprinted and X-linked genes from bulk and single-cell transcriptomes.Communications biology · 2022Article
- Epigenetic Mechanisms of ART-Related Imprinting Disorders: Lessons From iPSC and Mouse Models.Genes · 2021Review
- hiPSCs for predictive modelling of neurodegenerative diseases: dreaming the possible.Nature reviews. Neurology · 2021Review
- The effects of the DNA Demethylating reagent, 5-azacytidine on SMCHD1 genomic localization.BMC genetics · 2020Article
- Expression of miRNAs from the ImprintedCells · 2019Article
- How Does Reprogramming to Pluripotency Affect Genomic Imprinting?Frontiers in cell and developmental biology · 2019Review
- Concise Review: Molecular Cytogenetics and Quality Control: Clinical Guardians for Pluripotent Stem Cells.Stem cells translational medicine · 2018Review
- Transflammation: How Innate Immune Activation and Free Radicals Drive Nuclear Reprogramming.Antioxidants & redox signaling · 2018Review
- Chondrocytes differentiated from human induced pluripotent stem cells: Response to ionizing radiation.PloS one · 2018Article
- Transflammation: Innate immune signaling in nuclear reprogramming.Advanced drug delivery reviews · 2017Review
- Stem Cell Therapies for Treatment of Liver Disease.Biomedicines · 2016Review
- Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions.Stem cell research & therapy · 2015Article
- Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 region.Stem cell research & therapy · 2015Article
- Epigenetic mechanisms of induced pluripotency.Contemporary oncology (Poznan, Poland) · 2015Review
- A practical guide to induced pluripotent stem cell research using patient samples.Laboratory investigation; a journal of technical methods and pathology · 2015Review
- Genomic imprinting in development, growth, behavior and stem cells.Development (Cambridge, England) · 2014Review
- High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells.Cell research · 2014Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundhiPSCs are generated through epigenetic reprogramming of somatic tissue. Genomic imprinting is an epigenetic phenomenon through which monoallelic gene expression is regulated in a parent-of-origin-specific manner. Reprogramming relies on the successful erasure of marks of differentiation while maintaining those required for genomic imprinting. Loss of imprinting (LOI), which occurs in many types of malignant tumors, would hinder the clinical application of hiPSCs.
resultsWe examined the imprinting status, expression levels and DNA methylation status of eight imprinted genes in five independently generated hiPSCs. We found a low frequency of LOI in some lines. Where LOI was identified in an early passage cell line, we found that this was maintained through subsequent passages of the cells. Just as normal imprints are maintained in long-term culture, this work suggests that abnormal imprints are also stable in culture.
conclusionsAnalysis of genomic imprints in hiPSCs is a necessary safety step in regenerative medicine, with relevance both to the differentiation potential of these stem cells and also their potential tumorigenic properties.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.