ArticleBMC genetics2013
Identification of Lens culinaris defense genes responsive to the anthracnose pathogen Colletotrichum truncatum.
Article in BMC genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Conventional and new-breeding technologies for improving disease resistance in lentil (Frontiers in plant science · 2022Pooled it
- Gene co-expression analysis reveals conserved and distinct gene networks between resistant and susceptible Lens ervoides challenged by hemibiotrophic and necrotrophic pathogens.Scientific reports · 2024Article
- Omics-Driven Strategies for Developing Saline-Smart Lentils: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Morphological and transcriptional analysis of Colletotrichum lindemuthianum race 7 during early stages of infection in common bean.Genetics and molecular biology · 2024Article
- Construction and genetic characterization of an interspecific raspberry hybrids panel aiming resistance to late leaf rust and adaptation to tropical regions.Scientific reports · 2023Article
- Identification of candidate genes associated with resistance against race 0 of Colletotrichum lentis in Lens ervoides.Scientific reports · 2022Article
- A successful defense of the narrow-leafed lupin against anthracnose involves quick and orchestrated reprogramming of oxidation-reduction, photosynthesis and pathogenesis-related genes.Scientific reports · 2022Article
- Chickpea Roots Undergoing Colonisation byMicroorganisms · 2022Article
- Article
- Comparative Transcriptome Analysis to Identify Candidate Genes forFrontiers in genetics · 2021Article
- Diversity, structure, and synteny of the cutinase gene ofEcology and evolution · 2020Article
- Genetic map-guided genome assembly reveals a virulence-governing minichromosome in the lentil anthracnose pathogen Colletotrichum lentis.The New phytologist · 2019Article
- Validation of reference genes for qRT-PCR data normalisation in lentil (Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Transcriptional analysis and histochemistry reveal that hypersensitive cell death and HHorticulture research · 2018Article
- Biotrophy-necrotrophy switch in pathogen evoke differential response in resistant and susceptible sesame involving multiple signaling pathways at different phases.Scientific reports · 2017Article
- Transcriptome analysis reveals a complex interplay between resistance and effector genes during the compatible lentil-Colletotrichum lentis interaction.Scientific reports · 2017Article
- Genotype-Dependent Interaction of Lentil Lines withFrontiers in plant science · 2017Article
- Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundAnthracnose of lentil, caused by the hemibiotrophic fungal pathogen Colletotrichum truncatum is a serious threat to lentil production in western Canada. Colletotrichum truncatum employs a bi-phasic infection strategy characterized by initial symptomless biotrophic and subsequent destructive necrotrophic colonization of its host. The transition from biotrophy to necrotrophy (known as the biotrophy-necrotrophy switch [BNS]) is critical in anthracnose development. Understanding plant responses during the BNS is the key to designing a strategy for incorporating resistance against hemibiotrophic pathogens either via introgression of resistance genes or quantitative trait loci contributing to host defense into elite cultivars, or via incorporation of resistance by biotechnological means.
resultsThe in planta BNS of C. truncatum was determined by histochemical analysis of infected lentil leaf tissues in time-course experiments. A total of 2852 lentil expressed sequence tags (ESTs) derived from C. truncatum-infected leaf tissues were analyzed to catalogue defense related genes. These ESTs could be assembled into 1682 unigenes. Of these, 101 unigenes encoded membrane and transport associated proteins, 159 encoded proteins implicated in signal transduction and 387 were predicted to be stress and defense related proteins (GenBank accessions: JG293480 to JG293479). The most abundant class of defense related proteins contained pathogenesis related proteins (encoded by 125 ESTs) followed by heat shock proteins, glutathione S-transferase, protein kinases, protein phosphatase, zinc finger proteins, peroxidase, GTP binding proteins, resistance proteins and syringolide-induced proteins. Quantitative RT-PCR was conducted to compare the expression of two resistance genes of the NBS-LRR class in susceptible and partially resistant genotypes. One (contig186) was induced 6 days post-inoculation (dpi) in a susceptible host genotype (Eston) whereas the mRNA level of another ( LT21-1990) peaked 4 dpi in a partially resistant host genotype (Robin), suggesting roles in conditioning the susceptibility and conferring tolerance to the pathogen, respectively.
conclusionsData obtained in this study suggest that lentil cells recognize C. truncatum at the BNS and in response, mount an inducible defense as evident by a high number of transcripts (23% of the total pathogen-responsive lentil transcriptome) encoding defense related proteins. Temporal expression polymorphism of defense related genes could be used to distinguish the response of a lentil genotype as susceptible or resistant.
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