ArticleJournal of immunology (Baltimore, Md. : 1950)2013
The restricted DH gene reading frame usage in the expressed human antibody repertoire is selected based upon its amino acid content.
Article in Journal of immunology (Baltimore, Md. : 1950), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- VH-replacement shapes the antibody repertoire by removing the genes of non-functional heavy-chains.The EMBO journal · 2025Article
- Unveiling inverted D genes and D-D fusions in human antibody repertoires unlocks novel antibody diversity.Communications biology · 2025Article
- Modulating the immunodominance hierarchy of immunoglobulin germline-encoded structural motifs targeting the influenza hemagglutinin stem.Cell reports · 2024Article
- A single donor is sufficient to produce a highly functional in vitro antibody library.Communications biology · 2021Article
- Landscape of Non-canonical Cysteines in Human VCell reports · 2020Article
- The Bayesian optimist's guide to adaptive immune receptor repertoire analysis.Immunological reviews · 2018Review
- On being the right size: antibody repertoire formation in the mouse and human.Immunogenetics · 2018Review
- Evidence for Shaping of Light Chain Repertoire by Structural Selection.Frontiers in immunology · 2018Article
- Many Routes to an Antibody Heavy-Chain CDR3: Necessary, Yet Insufficient, for Specific Binding.Frontiers in immunology · 2018Article
- Estimate of within population incremental selection through branch imbalance in lineage trees.Nucleic acids research · 2016Article
- B cell development in chromosome 22q11.2 deletion syndrome.Clinical immunology (Orlando, Fla.) · 2016Article
- Consistency of VDJ Rearrangement and Substitution Parameters Enables Accurate B Cell Receptor Sequence Annotation.PLoS computational biology · 2016Article
- The analysis of clonal expansions in normal and autoimmune B cell repertoires.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- The promise and challenge of high-throughput sequencing of the antibody repertoire.Nature biotechnology · 2014Review
- The Antibody Germline/Maturation Hypothesis, Elicitation of Broadly Neutralizing Antibodies Against HIV-1 and Cord Blood IgM Repertoires.Frontiers in immunology · 2014Article
- The shape of the lymphocyte receptor repertoire: lessons from the B cell receptor.Frontiers in immunology · 2013Review
- Multi Step Selection in Ig H Chains is Initially Focused on CDR3 and Then on Other CDR Regions.Frontiers in immunology · 2013Article
- Review
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9 authors.
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Abstract
The Ab repertoire is not uniform. Some variable, diversity, and joining genes are used more frequently than others. Nonuniform usage can result from the rearrangement process, or from selection. To study how the Ab repertoire is selected, we analyzed one part of diversity generation that cannot be driven by the rearrangement mechanism: the reading frame usage of DH genes. We have used two high-throughput sequencing methodologies, multiple subjects and advanced algorithms to measure the DH reading frame usage in the human Ab repertoire. In most DH genes, a single reading frame is used predominantly, and inverted reading frames are practically never observed. The choice of a single DH reading frame is not limited to a single position of the DH gene. Rather, each DH gene participates in rearrangements of differing CDR3 lengths, restricted to multiples of three. In nonproductive rearrangements, there is practically no reading frame bias, but there is still a striking absence of inversions. Biases in DH reading frame usage are more pronounced, but also exhibit greater interindividual variation, in IgG(+) and IgA(+) than in IgM(+) B cells. These results suggest that there are two developmental checkpoints of DH reading frame selection. The first occurs during VDJ recombination, when inverted DH genes are usually avoided. The second checkpoint occurs after rearrangement, once the BCR is expressed. The second checkpoint implies that DH reading frames are subjected to differential selection. Following these checkpoints, clonal selection induces a host-specific DH reading frame usage bias.
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