ArticlePlant physiology2013
The methylation of the PcMYB10 promoter is associated with green-skinned sport in Max Red Bartlett pear.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
112 citing papers in PubMed, 256 citations in OpenAlex.
- Multifaceted mechanisms underlying species- and cultivar-associated differential anthocyanin accumulation in fruits.Horticulture research · 2026Article
- Overexpression of a Tonoplast Malate Transporter Gene Leads to Enhanced Anthocyanin Biosynthesis in Apple.Plant biotechnology journal · 2026Article
- The grape berry methylome reveals tissue-specific features associated with metabolism in ripening.Horticulture research · 2025Article
- Characterization of a KANADI-like transcription factor that suppresses pear anthocyanin biosynthesis.Horticulture research · 2025Article
- Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025Review
- Identification ofGenes · 2025Article
- Review
- Single-Base Methylome Analysis of Sweet Cherry (International journal of molecular sciences · 2024Article
- For a Colorful Life: Recent Advances in Anthocyanin Biosynthesis during Leaf Senescence.Biology · 2024Review
- Multi-omics provide insights into the regulation of DNA methylation in pear fruit metabolism.Genome biology · 2024Article
- High-density genetic map and quantitative trait loci map of skin color in hawthorn (Frontiers in genetics · 2024Article
- Identification of Flavanone 3-Hydroxylase Gene Family in Strawberry and Expression Analysis of Fruit at Different Coloring Stages.International journal of molecular sciences · 2023Article
- DNA cytosine methylation dynamics and functional roles in horticultural crops.Horticulture research · 2023Article
- Virus-Induced Gene Silencing in the Tea Plant (Plants (Basel, Switzerland) · 2023Article
- Advances in DNA methylation and demethylation in medicinal plants: a review.Molecular biology reports · 2023Review
- Colorful hues: insight into the mechanisms of anthocyanin pigmentation in fruit.Plant physiology · 2023Article
- The mechanisms underpinning anthocyanin accumulation in a red-skinned bud sport in pear (Pyrus ussuriensis).Plant cell reports · 2023Article
- The combination of DNA methylation and positive regulation of anthocyanin biosynthesis by MYB and bHLH transcription factors contributes to the petal blotch formation in Xibei tree peony.Horticulture research · 2023Article
- The MYB transcription factor RcMYB1 plays a central role in rose anthocyanin biosynthesis.Horticulture research · 2023Article
- Somatic Mutations in Fruit Trees: Causes, Detection Methods, and Molecular Mechanisms.Plants (Basel, Switzerland) · 2023Review
52 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Varieties of the European pear (Pyrus communis) can produce trees with both red- and green-skinned fruits, such as the Max Red Bartlett (MRB) variety, although little is known about the mechanism behind this differential pigmentation. In this study, we investigated the pigmentation of MRB and its green-skinned sport (MRB-G). The results suggest that a reduction in anthocyanin concentration causes the MRB-G sport. Transcript levels of PcUFGT (for UDP-glucose:flavonoid 3-O-glucosyltransferase), the key structural gene in anthocyanin biosynthesis, paralleled the change of anthocyanin concentration in both MRB and MRB-G fruit. We cloned the PcMYB10 gene, a transcription factor associated with the promoter of PcUFGT. An investigation of the 2-kb region upstream of the ATG translation start site of PcMYB10 showed the regions -604 to -911 bp and -1,218 to -1,649 bp to be highly methylated. A comparison of the PcMYB10 promoter methylation level between the MRB and MRB-G forms indicated a correlation between hypermethylation and the green-skin phenotype. An Agrobacterium tumefaciens infiltration assay was conducted on young MRB fruits by using a plasmid constructed to silence endogenous PcMYB10 via DNA methylation. The infiltrated fruits showed blocked anthocyanin biosynthesis, higher methylation of the PcMYB10 promoter, and lower expression of PcMYB10 and PcUFGT. We suggest that the methylation level of PcMYB10 is associated with the formation of the green-skinned sport in the MRB pear. The potential mechanism behind the regulation of anthocyanin biosynthesis is discussed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.